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富血小板血浆凝胶对兔模型全层软骨缺损修复的有效性。

The effectiveness of platelet-rich plasma gel on full-thickness cartilage defect repair in a rabbit model.

作者信息

Slimi Fathia, Zribi Wassim, Trigui Moez, Amri Raja, Gouiaa Nawrez, Abid Cyrine, Rebai Mohammed Ali, Boudawara Tahia, Jebahi Samira, Keskes Hassib

机构信息

Experimental Surgery of the Musculoskeletal System Laboratory, Faculty of Medicine, Sfax, Tunisia.

Department of Pathology, Habib Bourguiba Hospital, Sfax, Tunisia.

出版信息

Bone Joint Res. 2021 Mar;10(3):192-202. doi: 10.1302/2046-3758.103.BJR-2020-0087.R2.

DOI:10.1302/2046-3758.103.BJR-2020-0087.R2
PMID:33730862
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7998069/
Abstract

AIMS

The present study investigates the effectiveness of platelet-rich plasma (PRP) gel without adjunct to induce cartilage regeneration in large osteochondral defects in a rabbit model.

METHODS

A bilateral osteochondral defect was created in the femoral trochlear groove of 14 New Zealand white rabbits. The right knees were filled with PRP gel and the contralateral knees remained untreated and served as control sides. Some animals were killed at week 3 and others at week 12 postoperatively. The joints were harvested and assessed by Magnetic Resonance Observation of Cartilage Repair Tissue (MOCART) MRI scoring system, and examined using the International Cartilage Repair Society (ICRS) macroscopic and ICRS histological scoring systems. Additionally, the collagen type II content was evaluated by the immunohistochemical staining.

RESULTS

After 12 weeks post-surgery, the defects of the PRP group were repaired by hyaline cartilage-like tissue. However, incomplete cartilage regeneration was observed in the PRP group for three weeks. The control groups showed fibrocartilaginous or fibrous tissue, respectively, at each timepoint.

CONCLUSION

Our study proved that the use of PRP gel without any adjuncts could successfully produce a good healing response and resurface the osteochondral defect with a better quality of cartilage in a rabbit model. Cite this article:  2021;10(3):192-202.

摘要

目的

本研究探讨在兔模型中，无辅助剂的富血小板血浆（PRP）凝胶诱导大的骨软骨缺损处软骨再生的有效性。

方法

在14只新西兰白兔的股骨滑车沟制造双侧骨软骨缺损。右膝填充PRP凝胶，对侧膝关节不治疗作为对照。部分动物在术后3周处死，其余在术后12周处死。取出关节，采用磁共振观察软骨修复组织（MOCART）MRI评分系统进行评估，并使用国际软骨修复协会（ICRS）宏观和组织学评分系统进行检查。此外，通过免疫组织化学染色评估II型胶原蛋白含量。

结果

术后12周，PRP组缺损由透明软骨样组织修复。然而，PRP组在3周时观察到软骨再生不完全。对照组在每个时间点分别显示纤维软骨或纤维组织。

结论

我们的研究证明，在兔模型中，使用无任何辅助剂的PRP凝胶可成功产生良好的愈合反应，并用质量更好的软骨覆盖骨软骨缺损。引用本文：2021;10(3):192 - 202。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7c5/7998069/af0428918737/BJR-10-192-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7c5/7998069/995b71dee9ad/BJR-10-192-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7c5/7998069/03d984d7ae4d/BJR-10-192-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7c5/7998069/43b1007c7b04/BJR-10-192-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7c5/7998069/220545581395/BJR-10-192-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7c5/7998069/ca9f8c0882c0/BJR-10-192-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7c5/7998069/da69f42ecd0e/BJR-10-192-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7c5/7998069/af0428918737/BJR-10-192-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7c5/7998069/995b71dee9ad/BJR-10-192-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7c5/7998069/03d984d7ae4d/BJR-10-192-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7c5/7998069/43b1007c7b04/BJR-10-192-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7c5/7998069/220545581395/BJR-10-192-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7c5/7998069/ca9f8c0882c0/BJR-10-192-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7c5/7998069/da69f42ecd0e/BJR-10-192-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7c5/7998069/af0428918737/BJR-10-192-g0007.jpg

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