Schiffmann Christian L, Otto Wolfgang, Hansen Rasmus, Nielsen Per Halkjær, Adrian Lorenz, Seifert Jana, von Bergen Martin, Jehmlich Nico
Department of Molecular Systems Biology, Helmholtz Centre for Environmental Research-UFZ, Permoserstr. 15, 04318 Leipzig, Germany.
Aalborg University, Department of Biotechnology, Chemistry and Environmental Engineering, Sohngårdsholmsvej 49, 9000 Aalborg, Denmark.
Data Brief. 2016 Feb 22;7:253-6. doi: 10.1016/j.dib.2016.02.037. eCollection 2016 Jun.
The proteome of the anaerobic organohalide-respiring bacterium Dehalococcoides mccartyi strain CBDB1 was analyzed by nano liquid chromatography coupled to mass spectrometry (LC-MS/MS). Two different preparation methods, (i) in-solution and (ii) in-gel proteolytic digestion were assessed to elucidate the core and the functional proteome of bacterial cultures grown in synthetic anaerobic medium with hexachlorobenzene as sole electron acceptor. A detailed analysis of the data presented is available (Schiffmann et al., 2014) [1].
通过纳升液相色谱-质谱联用（LC-MS/MS）分析了厌氧有机卤化物呼吸细菌麦氏脱卤球菌菌株CBDB1的蛋白质组。评估了两种不同的制备方法，即（i）溶液内和（ii）胶内蛋白酶解，以阐明在以六氯苯作为唯一电子受体的合成厌氧培养基中生长的细菌培养物的核心蛋白质组和功能蛋白质组。已提供对所呈现数据的详细分析（Schiffmann等人，2014年）[1]。
