Engel P, Dischinger H C, Ullah A H
Southern Regional Research Laboratory, USDA, New Orleans, Louisiana 70124.
Prep Biochem. 1989;19(4):321-8. doi: 10.1080/10826068908544920.
A reversed-phase, C-18 HPLC method for separation, with baseline resolution, of the chitin synthase inhibitors nikkomycin X and Z is described. This permits, for the first time, satisfactory identification of nikkomycin X and Z contained in a mixture. The use of 30 mM ammonium formate (pH 4.7) containing the ion-pair agent heptanesulfonic acid (1 mM) was critical for the successful separation of these fungicides.
