• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

通过基质辅助激光解吸电离质谱成像技术观察真菌寄生相互作用过程中的真菌代谢产物。

Visualizing fungal metabolites during mycoparasitic interaction by MALDI mass spectrometry imaging.

作者信息

Holzlechner Matthias, Reitschmidt Sonja, Gruber Sabine, Zeilinger Susanne, Marchetti-Deschmann Martina

机构信息

Institute of Chemical Technologies and Analytics, Vienna University of Technology, Vienna, Austria.

Institute of Microbiology, University of Innsbruck, Innsbruck, Austria.

出版信息

Proteomics. 2016 Jun;16(11-12):1742-6. doi: 10.1002/pmic.201500510. Epub 2016 Apr 13.

DOI:10.1002/pmic.201500510
PMID:26959280
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4982067/
Abstract

Studying microbial interactions by MALDI mass spectrometry imaging (MSI) directly from growing media is a difficult task if high sensitivity is demanded. We present a quick and robust sample preparation strategy for growing fungi (Trichoderma atroviride, Rhizoctonia solani) on glass slides to establish a miniaturized confrontation assay. By this we were able to visualize metabolite distributions by MALDI MSI after matrix deposition with a home-built sublimation device and thorough recrystallization. We present for the first time MALDI MSI data for secondary metabolite release during active mycoparasitism.

摘要

如果需要高灵敏度，通过基质辅助激光解吸电离质谱成像（MSI）直接从生长培养基中研究微生物相互作用是一项艰巨的任务。我们提出了一种快速且稳健的样品制备策略，用于在载玻片上培养真菌（深绿木霉、立枯丝核菌），以建立小型化对峙试验。通过这种方法，我们能够在使用自制升华装置沉积基质并进行彻底重结晶后，通过MALDI MSI可视化代谢物分布。我们首次展示了在活跃的真菌寄生过程中次生代谢物释放的MALDI MSI数据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f293/4982067/45d09603fef2/PMIC-16-1742-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f293/4982067/0979a4e7ea16/PMIC-16-1742-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f293/4982067/45d09603fef2/PMIC-16-1742-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f293/4982067/0979a4e7ea16/PMIC-16-1742-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f293/4982067/45d09603fef2/PMIC-16-1742-g002.jpg

相似文献

1
Visualizing fungal metabolites during mycoparasitic interaction by MALDI mass spectrometry imaging.通过基质辅助激光解吸电离质谱成像技术观察真菌寄生相互作用过程中的真菌代谢产物。
Proteomics. 2016 Jun;16(11-12):1742-6. doi: 10.1002/pmic.201500510. Epub 2016 Apr 13.
2
Improvement of Sensitivity and Reproducibility for Imaging of Endogenous Metabolites by Matrix-Assisted Laser Desorption/Ionization-Mass Spectrometry.基质辅助激光解吸/电离质谱法提高内源性代谢物成像的灵敏度和重现性。
J Am Soc Mass Spectrom. 2019 Aug;30(8):1512-1520. doi: 10.1007/s13361-019-02221-7. Epub 2019 May 1.
3
The histone deacetylase Hda1 affects oxidative and osmotic stress response as well as mycoparasitic activity and secondary metabolite biosynthesis in .组蛋白去乙酰化酶 Hda1 影响酿酒酵母的氧化应激和渗透应激反应，以及其对其他真菌的寄生活性和次生代谢产物生物合成。
Microbiol Spectr. 2024 Mar 5;12(3):e0309723. doi: 10.1128/spectrum.03097-23. Epub 2024 Feb 9.
4
Argonaute and Dicer are essential for communication between and fungal hosts during mycoparasitism.Argonaute 和 Dicer 在菌寄生过程中对于 和真菌宿主之间的交流是必不可少的。
Microbiol Spectr. 2024 Apr 2;12(4):e0316523. doi: 10.1128/spectrum.03165-23. Epub 2024 Mar 5.
5
In Situ Analysis of Bacterial Lipopeptide Antibiotics by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging.基质辅助激光解吸/电离质谱成像技术对细菌脂肽抗生素的原位分析
Methods Mol Biol. 2016;1401:161-73. doi: 10.1007/978-1-4939-3375-4_11.
6
Mycoparasitism related targets of Tmk1 indicate stimulating regulatory functions of this MAP kinase in Trichoderma atroviride.Tmk1 相关的菌寄生作用靶标表明了该丝裂原活化蛋白激酶在深绿木霉中的刺激调控功能。
Sci Rep. 2023 Nov 15;13(1):19976. doi: 10.1038/s41598-023-47027-6.
7
Spatial Metabolite Profiling by Matrix-Assisted Laser Desorption Ionization Mass Spectrometry Imaging.基于基质辅助激光解吸电离质谱成像的空间代谢物分析
Adv Exp Med Biol. 2017;965:291-321. doi: 10.1007/978-3-319-47656-8_12.
8
Identification of mycoparasitism-related genes in Trichoderma atroviride.鉴定深绿木霉中的菌寄生相关基因。
Appl Environ Microbiol. 2011 Jul;77(13):4361-70. doi: 10.1128/AEM.00129-11. Epub 2011 Apr 29.
9
The G protein alpha subunit Tga1 of Trichoderma atroviride is involved in chitinase formation and differential production of antifungal metabolites.深绿木霉的G蛋白α亚基Tga1参与几丁质酶的形成以及抗真菌代谢产物的差异产生。
Fungal Genet Biol. 2005 Sep;42(9):749-60. doi: 10.1016/j.fgb.2005.04.009.
10
Transcriptomic response of the mycoparasitic fungus Trichoderma atroviride to the presence of a fungal prey.对真菌捕食者存在的反应转录组学的研究：木霉属真菌。
BMC Genomics. 2009 Nov 30;10:567. doi: 10.1186/1471-2164-10-567.

引用本文的文献

1
Regulation of biotic interactions and responses to abiotic stresses by MAP kinase pathways in plant pathogenic fungi.植物病原真菌中丝裂原活化蛋白激酶途径对生物相互作用的调控及对非生物胁迫的响应
Stress Biol. 2021 Aug 18;1(1):5. doi: 10.1007/s44154-021-00004-3.
2
Dissecting the brain with spatially resolved multi-omics.利用空间分辨多组学技术剖析大脑。
J Pharm Anal. 2023 Jul;13(7):694-710. doi: 10.1016/j.jpha.2023.04.003. Epub 2023 Apr 10.
3
MALDI Mass Spectrometry Imaging: A Potential Game-Changer in a Modern Microbiology.基质辅助激光解吸电离质谱成像技术：现代微生物学的潜在变革者。

本文引用的文献

1
The peptaibiotics database--a comprehensive online resource.肽抗生素数据库——一个全面的在线资源。
Chem Biodivers. 2015 May;12(5):743-51. doi: 10.1002/cbdv.201400393.
2
Peptaibol, secondary-metabolite, and hydrophobin pattern of commercial biocontrol agents formulated with species of the Trichoderma harzianum complex.哈茨木霉复合体物种配制的商业生物防治剂的肽菌素、次生代谢产物和疏水蛋白模式。
Chem Biodivers. 2015 Apr;12(4):662-84. doi: 10.1002/cbdv.201400300.
3
Screening the biosphere: the fungicolous fungus Trichoderma phellinicola, a prolific source of hypophellins, new 17-, 18-, 19-, and 20-residue peptaibiotics.
Cells. 2022 Dec 2;11(23):3900. doi: 10.3390/cells11233900.
4
Characterisation of the Antibiotic Profile of AZ78, an Effective Biological Control Agent of Plant Pathogenic Microorganisms.植物病原微生物有效生物防治剂AZ78的抗生素特性分析
Microorganisms. 2021 Jun 17;9(6):1320. doi: 10.3390/microorganisms9061320.
5
Effect of MALDI matrices on lipid analyses of biological tissues using MALDI-2 postionization mass spectrometry.基质效应对 MALDI-2 后电离质谱分析生物组织脂质的影响。
J Mass Spectrom. 2020 Dec;55(12):e4663. doi: 10.1002/jms.4663.
6
The Lipoxygenase Lox1 Is Involved in Light- and Injury-Response, Conidiation, and Volatile Organic Compound Biosynthesis in the Mycoparasitic Fungus .脂氧合酶Lox1参与了真菌寄生真菌的光反应、损伤反应、分生孢子形成和挥发性有机化合物生物合成。
Front Microbiol. 2020 Aug 27;11:2004. doi: 10.3389/fmicb.2020.02004. eCollection 2020.
7
Bioactive Secondary Metabolites from spp. against Phytopathogenic Fungi.来自[物种名称]的生物活性次级代谢产物对植物病原真菌的作用
Microorganisms. 2020 May 29;8(6):817. doi: 10.3390/microorganisms8060817.
8
Changes in Peptaibol Production of Species during In Vitro Antagonistic Interactions with Fungal Plant Pathogens.种间肽类抗生素产生的变化在与真菌植物病原体的体外拮抗相互作用过程中。
Biomolecules. 2020 May 7;10(5):730. doi: 10.3390/biom10050730.
9
Fluorinated Gold Nanoparticles for Nanostructure Imaging Mass Spectrometry.氟化金纳米粒子用于纳米结构成像质谱。
ACS Nano. 2018 Jul 24;12(7):6938-6948. doi: 10.1021/acsnano.8b02376. Epub 2018 Jul 6.
10
Mass Spectrometry Imaging: A Review of Emerging Advancements and Future Insights.质谱成像：新兴进展与未来展望综述
Anal Chem. 2018 Jan 2;90(1):240-265. doi: 10.1021/acs.analchem.7b04733. Epub 2017 Dec 13.
筛选生物群：真菌胶膜革菌，一个丰富的低丙氨酸衍生缩肽的来源，新的 17-、18-、19-和 20 残基缩肽抗生素。
Chem Biodivers. 2013 May;10(5):787-812. doi: 10.1002/cbdv.201200339.
4
MALDI-FTICR MS imaging as a powerful tool to identify Paenibacillus antibiotics involved in the inhibition of plant pathogens.基质辅助激光解吸电离飞行时间质谱成像作为一种强大的工具，可用于鉴定参与抑制植物病原体的类芽孢杆菌抗生素。
J Am Soc Mass Spectrom. 2013 Aug;24(8):1202-13. doi: 10.1007/s13361-013-0620-2. Epub 2013 May 1.
5
Imaging mass spectrometry in microbiology.微生物成像质谱技术。
Nat Rev Microbiol. 2011 Aug 8;9(9):683-94. doi: 10.1038/nrmicro2634.
6
Matrix sublimation/recrystallization for imaging proteins by mass spectrometry at high spatial resolution.基质升华/重结晶用于高空间分辨率质谱成像蛋白质。
Anal Chem. 2011 Jul 15;83(14):5728-34. doi: 10.1021/ac200998a. Epub 2011 Jun 28.
7
Translational research on Trichoderma: from 'omics to the field.从组学到田间：木霉的转化研究。
Annu Rev Phytopathol. 2010;48:395-417. doi: 10.1146/annurev-phyto-073009-114314.
8
Profiling of trichorzianines in culture samples of Trichoderma atroviride by liquid chromatography/tandem mass spectrometry.利用液相色谱/串联质谱法分析深绿木霉培养样品中的木霉素
Rapid Commun Mass Spectrom. 2007;21(24):3963-70. doi: 10.1002/rcm.3301.
9
Intact-cell MALDI-TOF mass spectrometry analysis of peptaibol formation by the genus Trichoderma/Hypocrea: can molecular phylogeny of species predict peptaibol structures?木霉属/肉座菌属形成缩氨酸环肽的完整细胞基质辅助激光解吸电离飞行时间质谱分析：物种的分子系统发育能否预测缩氨酸环肽结构？
Microbiology (Reading). 2007 Oct;153(Pt 10):3417-3437. doi: 10.1099/mic.0.2007/006692-0.
10
Facts and challenges in the understanding of the biosynthesis of peptaibols by Trichoderma.
Chem Biodivers. 2007 Jun;4(6):1068-82. doi: 10.1002/cbdv.200790097.