用于分子流行病学和分类学的乙酰氨基芴标记核糖体RNA
Acetylaminofluorene-labelled ribosomal RNA for use in molecular epidemiology and taxonomy.
作者信息
Grimont F, Chevrier D, Grimont P A, Lefevre M, Guesdon J L
机构信息
Unité 199 INSERM, Institut Pasteur, Paris.
出版信息
Res Microbiol. 1989 Sep;140(7):447-54. doi: 10.1016/0923-2508(89)90065-x.
The use of acetylaminofluorene-labelled 16 + 23S rRNA (from Escherichia coli) is described for determining rRNA-gene-restriction patterns. The labelled probe allowed molecular fingerprinting of bacteria belonging to diverse phylogenetic branches (Enterobacteriaceae, Haemophilus, Pseudomonas, Acinetobacter, Brucella, Leptospira, Cytophaga, Campylobacter, Methylophaga). The labelled probe can be stored frozen (-20 degrees C) for at least a year and can endure vacuum dessication, ethanol precipitation or lyophilization.
描述了使用乙酰氨基芴标记的16 + 23S rRNA（来自大肠杆菌）来确定rRNA基因限制模式。该标记探针可对属于不同系统发育分支的细菌（肠杆菌科、嗜血杆菌属、假单胞菌属、不动杆菌属、布鲁氏菌属、钩端螺旋体属、噬纤维菌属、弯曲杆菌属、嗜甲基菌属）进行分子指纹识别。该标记探针可在冷冻条件下（-20摄氏度）保存至少一年，并且能够经受真空干燥、乙醇沉淀或冻干处理。
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