Altwegg M, Mayer L W
Division of Bacterial Diseases, Centers for Disease Control, Atlanta, GA 30333.
Res Microbiol. 1989 May-Jun;140(4-5):325-33. doi: 10.1016/0923-2508(89)90024-7.
The use of plasmid pKK3535 (a pBR322-derived plasmid containing a ribosomal RNA operon) is described for determining rRNA gene restriction patterns. The plasmid allowed the use of both radioactive as well as nonradioactive procedures and provided features that are necessary for the inclusion of internal and/or external standards that facilitate comparison of strains. The patterns generated are identical to those obtained by hybridization with 32P-labelled rRNA.
描述了使用质粒pKK3535（一种源自pBR322的质粒，含有一个核糖体RNA操纵子）来确定rRNA基因限制图谱。该质粒允许使用放射性以及非放射性方法，并提供了纳入内部和/或外部标准以促进菌株比较所需的特性。所产生的图谱与用32P标记的rRNA杂交获得的图谱相同。
