Kim Ji-Young, Yoo Han-Su, Lee Dong-Heon, Park So-Hyun, Kim Young-Ju, Oh Duck-Chul
Department of Biology, Jeju National University, Jeju 690-756, Republic of Korea.
Jeju Biological Resource Co., Ltd, CTC Business Incubator Jeju Tourism College, Jeju 690-756, Republic of Korea.
Int J Syst Evol Microbiol. 2016 Jun;66(6):2218-2224. doi: 10.1099/ijsem.0.001014. Epub 2016 Mar 9.
A Gram-stain-negative, aerobic, rod-shaped bacterium motile by means of a single polar flagella, strain ST-6T, was isolated from a brown alga (Sargassum thunbergii) collected in Jeju, Republic of Korea. Strain ST-6T was psychrotolerant, growing at 4-30 °C (optimum 20 °C). Phylogenetic analysis based on 16S rRNA and gyrB gene sequences revealed that strain ST-6T belonged to a distinct lineage in the genus Shewanella. Strain ST-6T was related most closely to Shewanella basaltis J83T, S. gaetbuli TF-27T, S. arctica IT12T, S. vesiculosa M7T and S. aestuarii SC18T, showing 96-97 % and 85-70 % 16S rRNA and gyrB gene sequences similarities, respectively. DNA-DNA relatedness values between strain ST-6T and the type strains of two species of the genus Shewanella were <22.6 %. The major cellular fatty acids (>5 %) were summed feature 3 (comprising C16:1ω7c and/ or iso-C15:0 2-OH), C16:0, iso-C13:0 and C17:1ω8c. The DNA G+C content of strain ST-6Twas 42.4 mol%, and the predominant isoprenoid quinones were menaquinone MK-7 and ubiquinones Q-7 and Q-8. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain ST-6T is considered to represent a novel species of the genus Shewanella, for which the name Shewanella algicola sp. nov. is proposed. The type strain is ST-6T (= KCTC 23253T = JCM 31091T).
从韩国济州岛采集的一种褐藻（鼠尾藻）中分离出一株革兰氏阴性、需氧、杆状细菌，菌株ST-6T，通过单根极生鞭毛运动。菌株ST-6T具有耐冷性，在4-30°C（最适温度20°C）下生长。基于16S rRNA和gyrB基因序列的系统发育分析表明，菌株ST-6T属于希瓦氏菌属中的一个独特谱系。菌株ST-6T与玄武希瓦氏菌J83T、加特希瓦氏菌TF-27T、北极希瓦氏菌IT12T、泡囊希瓦氏菌M7T和河口希瓦氏菌SC18T关系最为密切，16S rRNA和gyrB基因序列相似性分别为96-97%和85-70%。菌株ST-6T与希瓦氏菌属两个种的模式菌株之间的DNA-DNA杂交值<22.6%。主要细胞脂肪酸（>5%）为总和特征3（包含C16:1ω7c和/或异-C15:0 2-OH）、C16:0、异-C13:0和C17:1ω8c。菌株ST-6T的DNA G+C含量为42.4 mol%，主要类异戊二烯醌为甲基萘醌MK-7以及泛醌Q-7和Q-8。基于其表型特征和系统发育独特性，菌株ST-6T被认为代表希瓦氏菌属的一个新物种，为此提议命名为嗜藻希瓦氏菌（Shewanella algicola sp. nov.）。模式菌株为ST-6T（=KCTC 23253T = JCM 31091T）。
