Chaudhary Anchal, Ketkar Omkar Avinash, Irfan Sayed, Rana Varnika, Rahi Praveen, Deshmukh Rupesh, Kaur Jagdeep, Dhar Hena
National Agri-Food Biotechnology Institute (NABI), Mohali 140306, India.
Department of Biotechnology, Panjab University, Chandigarh 160025, India.
Biology (Basel). 2022 Apr 21;11(5):632. doi: 10.3390/biology11050632.
The genus is widely distributed in niches ranging from an aquatic environment to spoiled fish and is loaded with various ecologically and commercially important metabolites. Bacterial species under this genus find application in bioelectricity generation and bioremediation due to their capability to use pollutants as the terminal electron acceptor and could produce health-beneficial omega-3 fatty acids, particularly eicosapentaenoic acid (EPA). Here, the genome sequence of an EPA-producing bacterium, sp. N2AIL, isolated from the gastrointestinal tract of Tilapia fish, is reported. The genome size of the strain was 4.8 Mb with a GC content of 46.3% containing 4385 protein-coding genes. Taxonogenomic analysis assigned this strain to the genus on the basis of average nucleotide identity (ANI) and in silico DNA-DNA hybridization (DDH), phylogenetically most closely related with . NCTC 10735. The comparative genome analysis with the type strain of . revealed 693 unique genes in the strain N2AIL, highlighting the variation at the strain level. The genes associated with stress adaptation, secondary metabolite production, antibiotic resistance, and metal reduction were identified in the genome suggesting the potential of the bacterium to be explored as an industrially important strain. PUFA synthase gene cluster of size ~20.5 kb comprising all the essential domains for EPA biosynthesis arranged in five ORFs was also identified in the strain N2AIL. The study provides genomic insights into the diverse genes of sp. N2AIL, which is particularly involved in adaptation strategies and prospecting secondary metabolite potential, specifically the biosynthesis of omega-3 polyunsaturated fatty acids.
该属广泛分布于从水生环境到变质鱼类的各种生态位中，并富含各种具有生态和商业重要性的代谢产物。该属中的细菌物种由于能够利用污染物作为终端电子受体，从而在生物发电和生物修复中得到应用，并且能够产生对健康有益的ω-3脂肪酸，特别是二十碳五烯酸（EPA）。在此，报道了从罗非鱼胃肠道分离出的产EPA细菌N2AIL菌株的基因组序列。该菌株的基因组大小为4.8 Mb，GC含量为46.3%，包含4385个蛋白质编码基因。分类基因组分析基于平均核苷酸同一性（ANI）和计算机模拟DNA-DNA杂交（DDH）将该菌株归类于该属，在系统发育上与NCTC 10735关系最为密切。与该属模式菌株的比较基因组分析揭示了N2AIL菌株中有693个独特基因，突出了菌株水平上的差异。在基因组中鉴定出了与应激适应、次生代谢产物产生、抗生素抗性和金属还原相关的基因，表明该细菌具有作为工业重要菌株进行探索的潜力。在N2AIL菌株中还鉴定出了大小约为20.5 kb的PUFA合酶基因簇，其包含在五个开放阅读框中排列的EPA生物合成的所有必需结构域。该研究提供了对N2AIL菌株多样基因的基因组见解，该菌株特别涉及适应策略和次生代谢产物潜力的勘探，特别是ω-3多不饱和脂肪酸的生物合成。
