Kim Ji-Young, Park So-Hyun, Seo Ga-Young, Kim Young-Ju, Oh Duck-Chul
Department of Biology, Jeju National University, Jeju 690-756, Republic of Korea.
Jeju Biological Resource Co., Ltd, CTC Business Incubator, Jeju Tourism College, Jeju 690-756, Republic of Korea.
Int J Syst Evol Microbiol. 2015 Sep;65(9):2791-2796. doi: 10.1099/ijs.0.000334. Epub 2015 May 15.
A novel bacterial strain, designated EC29(T), was isolated from the brown alga Ecklonia cava collected on Jeju Island, Republic of Korea. Cells of strain EC29(T) were Gram-stain-negative, aerobic, rod-shaped and motile by gliding. Growth was observed at 10-30 °C (optimum, 20-25 °C), at pH 6.0-9.5 (optimum, pH 7.5) and in the presence of 1-5% (w/v) NaCl. Phylogenetic analyses based on the 16S rRNA gene sequence revealed that the strain belonged to the genus Winogradskyella. Strain EC29(T) exhibited the highest 16S rRNA gene sequence similarities, of 96.5-97.8%, to the type strains of Winogradskyella pulchriflava EM106(T), Winogradskyella echinorum KMM 6211(T) and Winogradskyella ulvae KMM 6390(T). Strain EC29(T) exhibited < 27% DNA-DNA relatedness with Winogradskyella pulchriflava EM106(T) and Winogradskyella echinorum KMM 6211(T). The predominant fatty acids of strain EC29(T) were iso-C15 : 0, iso-C15 : 1 G, C15 : 0, iso-C17 : 0 3-OH, iso-C15 : 0 3-OH and anteiso-C15 : 0. The DNA G+C content was 31.1 mol% and the major respiratory quinone was menaquinone-6 (MK-6). Based on a polyphasic study, strain EC29(T) is considered to represent a novel species of the genus Winogradskyella, for which the name Winogradskyella eckloniae sp. nov. is proposed. The type strain is EC29(T) ( = KCTC 32172(T) = JCM 18703(T)).
从韩国济州岛采集的褐藻海蕴中分离出一株新的细菌菌株，命名为EC29(T)。菌株EC29(T)的细胞革兰氏染色阴性，好氧，杆状，通过滑行运动。在10-30℃（最适温度为20-25℃）、pH 6.0-9.5（最适pH为7.5）和1-5%（w/v）NaCl存在的条件下可观察到生长。基于16S rRNA基因序列的系统发育分析表明，该菌株属于维诺格拉德斯基氏菌属。菌株EC29(T)与美丽维诺格拉德斯基氏菌EM106(T)、棘维诺格拉德斯基氏菌KMM 6211(T)和海生维诺格拉德斯基氏菌KMM 6390(T)的模式菌株表现出最高的16S rRNA基因序列相似性，为96.5-97.8%。菌株EC29(T)与美丽维诺格拉德斯基氏菌EM106(T)和棘维诺格拉德斯基氏菌KMM 6211(T)的DNA-DNA相关性<27%。菌株EC29(T)的主要脂肪酸为异-C15 : 0、异-C15 : 1 G、C15 : 0、异-C17 : 0 3-OH、异-C15 : 0 3-OH和anteiso-C15 : 0。DNA G+C含量为31.1 mol%，主要呼吸醌为甲基萘醌-6（MK-6）。基于多相研究，菌株EC29(T)被认为代表维诺格拉德斯基氏菌属的一个新物种，为此提出名为海蕴维诺格拉德斯基氏菌（Winogradskyella eckloniae sp. nov.）。模式菌株为EC29(T)（=KCTC 32172(T)=JCM 18703(T)）。
