Vashisth Priya, Srivastava Amit Kumar, Nagar Hemant, Raghuwanshi Navdeep, Sharan Shruti, Nikhil Kumar, Pruthi Parul A, Singh Rajesh P, Roy Partha, Pruthi Vikas
Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, India.
Sapience Bio-Analytical Research Lab Bhopal, Madhya Pradesh, India.
Nanomedicine. 2016 Jul;12(5):1375-85. doi: 10.1016/j.nano.2016.01.019. Epub 2016 Mar 7.
In order to promote the natural healing process, drug-functionalized nanofibrous transdermal substitute was fabricated using gellan as chief polymer and polyvinyl alcohol (PVA) as supporting polymer via electrospinning technique. These fabricated nanofibers physiochemically mimic the extracellular matrix (ECM) which supports the cell growth. For neo-tissue regeneration in a sterilized environment, amoxicillin (Amx) was entrapped within these nanofibers. Entrapment of Amx in the nanofibers was confirmed by FESEM, FTIR, XRD and TG analysis. In vitro cell culture studies revealed that the fabricated non-cytotoxic nanofibers promoted enhance cell adherence and proliferation of human keratinocytes. A preliminary in vivo study performed on rat model for full thickness skin excision wound demonstrated the prompt re-epithelialization in early phase and quicker collagen deposition in later phases of wound healing in case of Amx-functionalized gellan/PVA nanofibers. Data collectively confirmed the potential usage of gellan based electrospun nanofibers as transdermal substitute for faster skin restoration.
为了促进自然愈合过程，以结冷胶为主要聚合物、聚乙烯醇（PVA）为辅助聚合物，通过静电纺丝技术制备了药物功能化纳米纤维透皮替代物。这些制备的纳米纤维在物理化学性质上模拟支持细胞生长的细胞外基质（ECM）。为了在无菌环境中实现新组织再生，将阿莫西林（Amx）包封在这些纳米纤维中。通过场发射扫描电子显微镜（FESEM）、傅里叶变换红外光谱（FTIR）、X射线衍射（XRD）和热重分析（TG）证实了Amx被包封在纳米纤维中。体外细胞培养研究表明，制备的无细胞毒性纳米纤维促进了人角质形成细胞的粘附和增殖。在大鼠全层皮肤切除伤口模型上进行的初步体内研究表明，在伤口愈合的早期，Amx功能化的结冷胶/PVA纳米纤维能迅速实现上皮再形成，在后期能更快地沉积胶原蛋白。这些数据共同证实了基于结冷胶的静电纺纳米纤维作为透皮替代物用于更快皮肤修复的潜在用途。
