Laosinwattana Chamroon, Somala Naphat, Dimak Jantra, Teerarak Montinee, Chotsaeng Nawasit
School of Agricultural Technology, King Mongkut's Institute of Technology Ladkrabang, Bangkok, 10520, Thailand.
School of Science, King Mongkut's Institute of Technology Ladkrabang, Bangkok, 10520, Thailand.
Sci Rep. 2025 Jan 25;15(1):3263. doi: 10.1038/s41598-025-87810-1.
A nanoemulsion was fabricated from Cananga odorata essential oil (EO) and stabilized by incorporation of Tween 80 using ultrasonication. The major constituents of the EO were benzyl benzoate, linalool, and phenylmethyl ester. Differing sonication amplitude (20-60%) and time (2-10 min) were assessed for effects on nanoemulsion droplet size and polydispersity index (PI). The smallest droplet size of 43.98 nm (PI 0.222) was obtained using 40% amplitude for 8 min; this nanoemulsion was evaluated for its droplet characteristics and pre-emergence herbicidal activities on Amaranthus tricolor. FT-IR confirmed ultrasonic emulsification to not affect the EO components. Regarding stability, storage at 4 °C was determined appropriate, with droplet size changing slightly after five weeks. Assays of herbicidal potential showed the coarse emulsion and nanoemulsion to both reduce A. tricolor germination and growth, with the nanoemulsion being more effective at a given concentration and the difference in effectivity correlating to droplet size. Remarkably, treatment with 250 ppm nanoemulsion and coarse emulsion respectively resulted in 100% and 63.75% germination inhibition. Both emulsions decreased seed imbibition and alpha-amylase activity. The highest relative electrolyte leakage was achieved in seed treated with the nanoemulsion. Therefore, this ultrasonic-based nanoemulsion may have utility as bioherbicide alternative.
以依兰香精油（EO）为原料，通过超声处理加入吐温80制备了纳米乳液。该精油的主要成分是苯甲酸苄酯、芳樟醇和苯甲酯。评估了不同的超声振幅（20 - 60%）和时间（2 - 10分钟）对纳米乳液液滴尺寸和多分散指数（PI）的影响。使用40%的振幅处理8分钟可获得最小液滴尺寸为43.98纳米（PI为0.222）；对该纳米乳液的液滴特性及其对三色苋的苗前除草活性进行了评估。傅里叶变换红外光谱（FT - IR）证实超声乳化不影响精油成分。关于稳定性，确定在4℃下储存是合适的，五周后液滴尺寸略有变化。除草潜力测定表明，粗乳液和纳米乳液均能降低三色苋的发芽率和生长，在给定浓度下纳米乳液更有效，且有效性差异与液滴尺寸相关。值得注意的是，分别用250 ppm的纳米乳液和粗乳液处理导致发芽抑制率分别为100%和63.75%。两种乳液均降低了种子吸胀和α -淀粉酶活性。用纳米乳液处理的种子相对电解质渗漏率最高。因此，这种基于超声的纳米乳液可能作为生物除草剂替代品具有实用性。
