Hardy Liselotte, Jespers Vicky, Abdellati Said, De Baetselier Irith, Mwambarangwe Lambert, Musengamana Viateur, van de Wijgert Janneke, Vaneechoutte Mario, Crucitti Tania
HIV and Sexual Health Group, Department of Public Health, Institute of Tropical Medicine, Antwerp, Belgium.
Laboratory Bacteriology Research, Faculty of Medicine & Health Sciences, University of Ghent, Ghent, Belgium.
Sex Transm Infect. 2016 Nov;92(7):487-491. doi: 10.1136/sextrans-2015-052475. Epub 2016 Mar 10.
Bacterial vaginosis (BV) is characterised by a change in the microbial composition of the vagina. The BV-associated organisms outnumber the health-associated species and form a polymicrobial biofilm on the vaginal epithelium, possibly explaining the difficulties with antibiotic treatment. A better understanding of vaginal biofilm with emphasis on and may contribute to a better diagnosis and treatment of BV.
To this purpose, we evaluated the association between the presence of both bacteria by fluorescence in situ hybridisation (FISH) and BV by Nugent scoring in 463 vaginal slides of 120 participants participating in a clinical trial in Rwanda.
A bacterial biofilm was detected in half of the samples using a universal bacterial probe. The biofilm contained in 54.1% and in 82.0% of the samples. was accompanied by in 99.5% of samples. The odds of having a Nugent score above 4 were increased for samples with dispersed and/or present (OR 4.5; CI 2 to 10.3). The probability of having a high Nugent score was even higher when a combination of adherent and dispersed was visualised (OR 75.6; CI 13.3 to 429.5) and highest when both bacteria were part of the biofilm (OR 119; CI 39.9 to 360.8).
Our study, although not comprehensive at studying the polymicrobial biofilm in BV, provided a strong indication towards the importance of and the symbiosis of and in this biofilm.
NCT01796613.
细菌性阴道病（BV）的特征是阴道微生物组成发生变化。与BV相关的微生物数量超过了与健康相关的物种，并在阴道上皮上形成多微生物生物膜，这可能解释了抗生素治疗的困难。更好地了解阴道生物膜，尤其是[此处原文缺失相关内容]，可能有助于更好地诊断和治疗BV。
为此，我们在卢旺达一项临床试验的120名参与者的463张阴道涂片上，通过荧光原位杂交（FISH）评估了两种细菌的存在与通过 Nugent评分评估的BV之间的关联。
使用通用细菌探针在一半的样本中检测到细菌生物膜。在54.1%的样本中生物膜含有[此处原文缺失相关内容]，在82.0%的样本中含有[此处原文缺失相关内容]。在99.5%的样本中，[此处原文缺失相关内容]伴随着[此处原文缺失相关内容]。对于存在分散的[此处原文缺失相关内容]和/或[此处原文缺失相关内容]的样本，Nugent评分高于4的几率增加（比值比4.5；可信区间2至10.3）。当观察到附着的[此处原文缺失相关内容]和分散的[此处原文缺失相关内容]同时存在时，Nugent高评分的概率更高（比值比75.6；可信区间13.3至429.5），而当两种细菌都是生物膜的一部分时概率最高（比值比119；可信区间39.9至360.8）。
我们的研究虽然在研究BV中的多微生物生物膜方面并不全面，但有力地表明了[此处原文缺失相关内容]以及[此处原文缺失相关内容]与[此处原文缺失相关内容]在该生物膜中的共生关系的重要性。
NCT01796613。
