Li Nuo, Kunitake Emi, Endo Yoshikazu, Aoyama Miki, Kanamaru Kyoko, Kimura Makoto, Kato Masashi, Kobayashi Tetsuo
a Department of Biological Mechanisms and Functions , Graduate School of Bioagricultural Sciences, Nagoya University , Nagoya , Japan.
Biosci Biotechnol Biochem. 2016 Sep;80(9):1820-8. doi: 10.1080/09168451.2016.1146074. Epub 2016 Mar 11.
SRF-MADS proteins are transcription factors conserved among eukaryotes that regulate a variety of cellular functions; however, their physiological roles are still not well understood in filamentous fungi. Effects of a mutation in mcmA gene that encodes the sole SRF-MADS protein in the fungus Aspergillus nidulans were examined by RNA sequencing. Sequencing data revealed that expression levels of cellulase genes were significantly decreased by the mutation as reported previously. However, expression levels of various hemicellulolytic enzyme genes, several extracellular protease genes, the nosA and rosA genes involved in sexual development, and AN4394 encoding an ortholog of EcdR involved in Aspergillus oryzae conidiation, were also significantly decreased by the mutation. As expected from the RNA sequencing data, the mcmA mutant had reduced protease production, cleistothecial development, and conidiation. This is the first report describing the involvement of SRF-MADS proteins in protease production in fungi, and asexual and sexual development in Aspergillus.
SRF-MADS蛋白是真核生物中保守的转录因子，可调节多种细胞功能；然而，它们在丝状真菌中的生理作用仍未得到充分了解。通过RNA测序研究了构巢曲霉中编码唯一SRF-MADS蛋白的mcmA基因突变的影响。测序数据显示，如先前报道，纤维素酶基因的表达水平因该突变而显著降低。然而，各种半纤维素分解酶基因、几个细胞外蛋白酶基因、参与有性发育的nosA和rosA基因，以及编码参与米曲霉分生孢子形成的EcdR直系同源物的AN4394的表达水平，也因该突变而显著降低。正如RNA测序数据所预期的那样，mcmA突变体的蛋白酶产量、闭囊壳发育和分生孢子形成减少。这是第一份描述SRF-MADS蛋白参与真菌蛋白酶产生以及曲霉无性和有性发育的报告。
