Murphy A, Casey A, Byrne G, Chambers G, Howe O
Nanolab Research Centre, Focas Research Institute, Dublin Institute of Technology, Camden Row, Dublin, 8, Ireland.
School of Biological Sciences, College of Sciences and Health, DIT, Kevin Street, Dublin, 8, Ireland.
J Appl Toxicol. 2016 Oct;36(10):1311-20. doi: 10.1002/jat.3315. Epub 2016 Mar 10.
A complete cytotoxic profile of exposure to silver (AgNP) nanoparticles investigating their biological effects on the innate immune response of circulating white blood cells is required to form a complete understanding of the risk posed. This was explored by measuring AgNP-stimulated gene expression of the pro-inflammatory cytokines interleukin-1 (IL-1), interleukin-6 (IL-6) and tumour necrosis factor-alpha (TNF-α) in THP-1 monocytes. A further study, on human monocytes extracted from a cohort of blood samples, was carried out to compare with the AgNP immune response in THP-1 cells along with the detection of pro-IL-1β which is a key mediator of the inflammasome complex. The aims of the study were to clearly demonstrate that AgNP can significantly up-regulate pro-inflammatory cytokine gene expression of IL-1, IL-6 and TNF-α in both THP-1 cells and primary blood monocytes thus indicating a rapid response to AgNP in circulation. Furthermore, a role for the inflammasome in AgNP response was indicated by pro-IL-1β cleavage and release. These results highlight the potential inflammatory effects of AgNP exposure and the responses evoked should be considered with respect to the potential harm that exposure may cause. Copyright © 2016 John Wiley & Sons, Ltd.
为了全面了解银（AgNP）纳米颗粒所带来的风险，需要对其暴露的完整细胞毒性概况进行研究，以探究它们对循环白细胞固有免疫反应的生物学影响。通过测量AgNP刺激的THP-1单核细胞中促炎细胞因子白细胞介素-1（IL-1）、白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）的基因表达来进行此项探索。另外，还对从一组血样中提取的人类单核细胞进行了研究，以与THP-1细胞中的AgNP免疫反应进行比较，并检测炎性小体复合物的关键介质前白细胞介素-1β。该研究的目的是明确证明AgNP可显著上调THP-1细胞和原代血单核细胞中IL-1、IL-6和TNF-α的促炎细胞因子基因表达，从而表明循环中对AgNP的快速反应。此外，前白细胞介素-1β的切割和释放表明炎性小体在AgNP反应中发挥了作用。这些结果突出了AgNP暴露的潜在炎症效应，并且应该考虑到暴露可能造成的潜在危害而引发的反应。版权所有© 2016约翰威立父子有限公司。
