Alghuthaymi Mousa A, Patil Sunita, Rajkuberan Chandrasekaran, Krishnan Muthukumar, Krishnan Ushani, Abd-Elsalam Kamel A
Biology Department, Science and Humanities College, Shaqra University, Alquwayiyah 11971, Saudi Arabia.
Rajiv Memorial Education Society's College of Pharmacy, Gulbarga 585102, India.
Plants (Basel). 2023 Mar 10;12(6):1261. doi: 10.3390/plants12061261.
Plant-mediated metallic nanoparticles have beenreported for a diversified range of applications in biological sciences. In the present study, we propose the flower as a reducing and stabilizing agent for the synthesis of silver nanoparticles (PTAgNPs). The PTAgNPs were exclusively characterized using UV-Visible spectroscopy, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), X-ray diffraction (XRD), atomic force microscopy, zeta potential, and transmission electron microscopy (TEM) studies. In a biological assay, we investigated the antibacterial and anticancer activity of silver nanoparticles in the A431 cell line. The PTAgNPs demonstrated a dose-dependent activity in and , suggesting the bactericidal nature of AgNPs. The PTAgNPs exhibited dose-dependent toxicity in the A431 cell line, with an IC of 54.56 µg/mL arresting cell growth at the S phase, as revealed by flow cytometry analysis. The COMET assay revealed 39.9% and 18.15 severities of DNA damage and tail length in the treated cell line, respectively. Fluorescence staining studies indicate that PTAgNPs cause reactive oxygen species (ROS) and trigger apoptosis. This research demonstrates that synthesized silver nanoparticles have a significant effect on inhibiting the growth of melanoma cells and other forms of skin cancer. The results show that these particles can cause apoptosis or cell death in malignant tumor cells. This suggests that they could be used to treat skin cancers without harming normal tissues.
植物介导的金属纳米颗粒已被报道在生物科学中有多种应用。在本研究中，我们提出将花朵作为合成银纳米颗粒（PTAgNPs）的还原剂和稳定剂。使用紫外可见光谱、傅里叶变换红外光谱（FTIR）、扫描电子显微镜（SEM）、X射线衍射（XRD）、原子力显微镜、zeta电位和透射电子显微镜（TEM）研究对PTAgNPs进行了专门表征。在生物测定中，我们研究了银纳米颗粒在A431细胞系中的抗菌和抗癌活性。PTAgNPs在[具体实验内容1]和[具体实验内容2]中表现出剂量依赖性活性，表明AgNPs具有杀菌性质。如流式细胞术分析所示，PTAgNPs在A431细胞系中表现出剂量依赖性毒性，IC50为54.56 µg/mL，可使细胞生长停滞在S期。彗星试验显示，处理后的细胞系中DNA损伤严重程度和尾巴长度分别为39.9%和18.15%。荧光染色研究表明，PTAgNPs会产生活性氧（ROS）并引发细胞凋亡。这项研究表明，合成的银纳米颗粒对抑制黑色素瘤细胞和其他形式的皮肤癌生长具有显著作用。结果表明，这些颗粒可导致恶性肿瘤细胞凋亡或死亡。这表明它们可用于治疗皮肤癌而不损害正常组织。
