Zhu Hongli, Xu Wenyan, Liu Hong, Wang Huaquan, Fu Rong, Wu Yuhong, Qu Wen, Wang Guojin, Guan Jing, Song Jia, Xing Limin, Shao Zonghong
Department of Hematology, General Hospital, Tianjin Medical University, 154 Anshandao, Heping District, Tianjin, 300052, China.
Int J Hematol. 2016 May;103(5):545-53. doi: 10.1007/s12185-016-1964-8. Epub 2016 Mar 11.
To investigate the expression of activation molecules on CD5(+)B lymphocytes in peripheral blood of autoimmune hemolytic anemia (AIHA)/Evans patients. The expression of CD80, CD86, and CD69 on CD5(+)B lymphocytes was detected using flow cytometry in 30 AIHA/Evans patients, 18 normal controls (NC) and nine chronic lymphocytic leukemia (CLL) patients. CD80 on CD5(+)B lymphocytes in untreated patients was higher than that in remission patients (P < 0.05), NC (P < 0.01) and CLL patients (P < 0.01). CD80 on CD5(+)B lymphocytes was higher than that on CD5(-)B lymphocytes in untreated patients (P > 0.05), but lower than those of CD5(-)B lymphocytes in remission patients and NC (P < 0.05). CD86 on CD5(+)B lymphocytes of untreated patients was higher than that of remission patients (P < 0.05), NC (P < 0.01). CD86 on CD5(+)B lymphocytes of CLL was higher than that of NC, remission (P < 0.05), and untreated patients (P > 0.05). CD80 and CD86 on CD5(+)B lymphocytes was negatively correlated with hemoglobin (HB), C3, C4 (P < 0.05) and positively correlated with reticulocyte (Ret) (P < 0.05). CD69 on CD5(+) and CD5(-)B lymphocytes of CLL was higher than those of AIHA/Evans patients and NC (P < 0.05). The active molecules on CD5(+)B lymphocytes in peripheral blood of AIHA/Evans patients differ from those on CD5(-) and clonal CD5(+)B lymphocytes.
探讨自身免疫性溶血性贫血（AIHA）/伊文氏综合征（Evans）患者外周血中CD5(+)B淋巴细胞上活化分子的表达情况。采用流式细胞术检测30例AIHA/Evans患者、18例正常对照（NC）及9例慢性淋巴细胞白血病（CLL）患者CD5(+)B淋巴细胞上CD80、CD86和CD69的表达。未治疗患者CD5(+)B淋巴细胞上的CD80高于缓解期患者（P<0.05）、NC（P<0.01）及CLL患者（P<0.01）。未治疗患者CD5(+)B淋巴细胞上的CD80高于CD5(-)B淋巴细胞（P>0.05），但低于缓解期患者及NC的CD5(-)B淋巴细胞（P<0.05）。未治疗患者CD5(+)B淋巴细胞上的CD86高于缓解期患者（P<0.05）、NC（P<0.01）。CLL患者CD5(+)B淋巴细胞上的CD86高于NC、缓解期患者（P<0.05）及未治疗患者（P>0.05）。CD5(+)B淋巴细胞上的CD80和CD86与血红蛋白（HB）、C3、C4呈负相关（P<0.05），与网织红细胞（Ret）呈正相关（P<0.05）。CLL患者CD5(+)和CD5(-)B淋巴细胞上的CD69高于AIHA/Evans患者及NC（P<0.05）。AIHA/Evans患者外周血CD5(+)B淋巴细胞上的活化分子与CD5(-)及克隆性CD5(+)B淋巴细胞上的不同。
