Baginsky Sacha
Institute of Biochemistry and Biotechnology, Martin-Luther-University Halle-Wittenberg, Weinbergweg 22, 06120 Halle (Saale), Germany
J Exp Bot. 2016 Jun;67(13):3873-82. doi: 10.1093/jxb/erw098. Epub 2016 Mar 11.
The development of new software tools, improved mass spectrometry equipment, a suite of optimized scan types, and better-quality phosphopeptide affinity capture have paved the way for an explosion of mass spectrometry data on phosphopeptides. Because phosphoproteomics achieves good sensitivity, most studies use complete cell extracts for phosphopeptide enrichment and identification without prior enrichment of proteins or subcellular compartments. As a consequence, the phosphoproteome of cell organelles often comes as a by-product from large-scale studies and is commonly assembled from these in meta-analyses. This review aims at providing some guidance on the limitations of meta-analyses that combine data from analyses with different scopes, reports on the current status of knowledge on chloroplast phosphorylation targets, provides initial insights into phosphorylation site conservation in different plant species, and highlights emerging information on the integration of gene expression with metabolism and photosynthesis by means of protein phosphorylation.
新软件工具的开发、改进的质谱设备、一系列优化的扫描类型以及质量更高的磷酸肽亲和捕获技术，为磷酸肽质谱数据的激增铺平了道路。由于磷酸化蛋白质组学具有良好的灵敏度，大多数研究使用完整的细胞提取物进行磷酸肽富集和鉴定，而无需事先对蛋白质或亚细胞区室进行富集。因此，细胞器的磷酸化蛋白质组通常作为大规模研究的副产品出现，并且通常在荟萃分析中由这些数据组装而成。本综述旨在为结合不同范围分析数据的荟萃分析的局限性提供一些指导，报告叶绿体磷酸化靶点的当前知识状态，初步了解不同植物物种中磷酸化位点的保守性，并突出通过蛋白质磷酸化将基因表达与代谢和光合作用整合的新信息。
