Xu Xiang, Yadav Nirbhay N, Song Xiaolei, McMahon Michael T, Jerschow Alexej, van Zijl Peter C M, Xu Jiadi
Russell H. Morgan Department of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, Baltimore, MD, United States; F.M. Kirby Research Center for Functional Brain Imaging, Kennedy Krieger Institute, Baltimore, MD, United States.
Russell H. Morgan Department of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, Baltimore, MD, United States; F.M. Kirby Research Center for Functional Brain Imaging, Kennedy Krieger Institute, Baltimore, MD, United States.
J Magn Reson. 2016 Apr;265:224-9. doi: 10.1016/j.jmr.2016.02.015. Epub 2016 Mar 4.
A chemical exchange saturation transfer (CEST) experiment can be performed in an ultrafast fashion if a gradient field is applied simultaneously with the saturation pulse. This approach has been demonstrated for studying dia- and para-magnetic CEST agents, hyperpolarized Xe gas and in vivo spectroscopy. In this study we present a simple method for the simultaneous screening of multiple samples. Furthermore, by interleaving a number of saturation and readout periods within the TR, a series of images with different saturation times can be acquired, allowing for the quantification of exchange rates using the variable saturation time (QUEST) approach in a much accelerated fashion, thus enabling high throughput screening of CEST contrast agents.
如果在施加饱和脉冲的同时施加梯度场，化学交换饱和转移（CEST）实验可以以超快方式进行。这种方法已被用于研究顺磁性和反磁性CEST试剂、超极化氙气以及体内光谱学。在本研究中，我们提出了一种同时筛选多个样品的简单方法。此外，通过在重复时间（TR）内交错多个饱和期和读出期，可以获取一系列具有不同饱和时间的图像，从而能够以更快的方式使用可变饱和时间（QUEST）方法对交换率进行量化，进而实现CEST造影剂的高通量筛选。
