Regional Centre of Advanced Technologies and Materials, Department of Physical Chemistry, Faculty of Science, Palacký University in Olomouc, 17. listopadu 12, 771 46 Olomouc, Czech Republic.
Regional Centre of Advanced Technologies and Materials, Department of Physical Chemistry, Faculty of Science, Palacký University in Olomouc, 17. listopadu 12, 771 46 Olomouc, Czech Republic.
Colloids Surf B Biointerfaces. 2016 Jun 1;142:392-399. doi: 10.1016/j.colsurfb.2016.03.007. Epub 2016 Mar 4.
Bacterial resistance to conventional antibiotics is currently one of the most important healthcare issues, and has serious negative impacts on medical practice. This study presents a potential solution to this problem, using the strong synergistic effects of antibiotics combined with silver nanoparticles (NPs). Silver NPs inhibit bacterial growth via a multilevel mode of antibacterial action at concentrations ranging from a few ppm to tens of ppm. Silver NPs strongly enhanced antibacterial activity against multiresistant, β-lactamase and carbapenemase-producing Enterobacteriaceae when combined with the following antibiotics: cefotaxime, ceftazidime, meropenem, ciprofloxacin and gentamicin. All the antibiotics, when combined with silver NPs, showed enhanced antibacterial activity at concentrations far below the minimum inhibitory concentrations (tenths to hundredths of one ppm) of individual antibiotics and silver NPs. The enhanced activity of antibiotics combined with silver NPs, especially meropenem, was weaker against non-resistant bacteria than against resistant bacteria. The double disk synergy test showed that bacteria produced no β-lactamase when treated with antibiotics combined with silver NPs. Low silver concentrations were required for effective enhancement of antibacterial activity against multiresistant bacteria. These low silver concentrations showed no cytotoxic effect towards mammalian cells, an important feature for potential medical applications.
细菌对传统抗生素的耐药性是当前最重要的医疗保健问题之一，对医疗实践产生了严重的负面影响。本研究提出了一种解决这一问题的潜在方法，即利用抗生素与银纳米粒子（NPs）的协同增效作用。银 NPs 通过从几 ppm 到几十 ppm 的浓度范围内的多种抗菌作用模式来抑制细菌生长。当与以下抗生素联合使用时，银 NPs 强烈增强了对多耐药、β-内酰胺酶和碳青霉烯酶产生的肠杆菌科的抗菌活性：头孢噻肟、头孢他啶、美罗培南、环丙沙星和庆大霉素。所有抗生素与银 NPs 联合使用时，在远低于单独抗生素和银 NPs 的最低抑菌浓度（十分之一到百分之一 ppm）的浓度下，均显示出增强的抗菌活性。抗生素与银 NPs 联合使用的增强活性，尤其是美罗培南，对非耐药菌的活性弱于对耐药菌的活性。双圆盘协同试验表明，用抗生素联合银 NPs 处理的细菌未产生β-内酰胺酶。有效增强对多耐药菌的抗菌活性需要较低的银浓度。这些低浓度的银对哺乳动物细胞没有细胞毒性作用，这是其在潜在医学应用中的一个重要特征。
