Barzegar Abolfazl, Ebrahimzadeh Somayyeh, Haeili Mehri, Shoghi Yalda, Ahmadi Alireza
Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran.
Molecular Medicine Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Biochem Biophys Rep. 2025 Jul 1;43:102121. doi: 10.1016/j.bbrep.2025.102121. eCollection 2025 Sep.
Nosocomial infections pose a significant threat to patient health and healthcare systems globally. This study explores the synergistic potential of green-synthesized NiFeO@Ag core-shell nanoparticles in enhancing the efficacy of conventional antibiotics against multidrug-resistant nosocomial pathogens. The NiFeO@Ag nanoparticles were synthesized using a green method and characterized by X-ray diffraction (XRD), revealing particle sizes of 36.4 nm for NiFeO and 51.54 nm for NiFeO@Ag. The antibacterial activity of these nanoparticles was evaluated alone and in combination with antibiotics-ciprofloxacin, tetracycline, and chloramphenicol-against five antibiotic-resistant bacterial strains associated with nosocomial infections, including Gram-negative , , , and , as well as Gram-positive . NiFeO@Ag nanoparticles exhibited potent antimicrobial activity, with minimum inhibitory concentration (MIC) values of 256-512 mg/L). Notably, the combination of NiFeO@Ag nanoparticles with chloramphenicol and ciprofloxacin resulted in fractional inhibitory concentration index (FICI) values ranging from 0.25 to 0.75, indicating significant synergistic or additive effects, against most of tested gram-negative pathogens. While combination of NiFeO@Ag with ciprofloxacin exhibited the strongest enhancement, no synergistic effects were observed using NiFeO@Ag nanoparticles and tetracycline combinations for most tested pathogens (except for ). The core-shell structure of NiFeO@Ag nanoparticles effectively integrates the magnetic properties of NiFeO with the antimicrobial activity of silver, enabling potential magnetic separation and reducing environmental impact. Additionally, the nanoparticles exhibited low cytotoxicity in HFF-2 cells, suggesting good biocompatibility. These findings highlight the potential of NiFeO@Ag nanoparticles as a promising therapeutic strategy to combat multidrug-resistant nosocomial pathogens.
医院感染对全球患者健康和医疗系统构成重大威胁。本研究探讨了绿色合成的NiFeO@Ag核壳纳米颗粒在增强传统抗生素对多重耐药医院病原体疗效方面的协同潜力。采用绿色方法合成了NiFeO@Ag纳米颗粒，并通过X射线衍射（XRD）对其进行了表征，结果显示NiFeO的粒径为36.4nm，NiFeO@Ag的粒径为51.54nm。单独评估了这些纳米颗粒以及它们与抗生素环丙沙星、四环素和氯霉素联合使用时对五种与医院感染相关的耐抗生素细菌菌株的抗菌活性，这些菌株包括革兰氏阴性菌、、、和，以及革兰氏阳性菌。NiFeO@Ag纳米颗粒表现出强大的抗菌活性，最低抑菌浓度（MIC）值为256 - 512mg/L）。值得注意的是，NiFeO@Ag纳米颗粒与氯霉素和环丙沙星联合使用时，对大多数测试的革兰氏阴性病原体的部分抑菌浓度指数（FICI）值在0.25至0.75之间，表明具有显著的协同或相加作用。虽然NiFeO@Ag与环丙沙星联合使用时增强作用最强，但对于大多数测试病原体（除外），NiFeO@Ag纳米颗粒与四环素联合使用时未观察到协同作用。NiFeO@Ag纳米颗粒的核壳结构有效地将NiFeO的磁性与银的抗菌活性结合在一起，实现了潜在的磁分离并减少了环境影响。此外，这些纳米颗粒在HFF - 2细胞中表现出低细胞毒性，表明具有良好的生物相容性。这些发现突出了NiFeO@Ag纳米颗粒作为对抗多重耐药医院病原体的一种有前景的治疗策略的潜力。
