State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, China.
State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, China.
J Colloid Interface Sci. 2016 Jun 1;471:20-28. doi: 10.1016/j.jcis.2016.03.009. Epub 2016 Mar 5.
Laccase is a green biocatalyst. It works with molecular oxygen and produces water as the only by-product. However, its practical application is far less than satisfactory due to the low stability/poor reusability of free laccase and the potential secondary pollution caused by dissolved mediators. To address those bottlenecks in laccase-based catalysis, a novel biocatalyst (Immo-LMS) was fabricated by simultaneously immobilizing both laccase and a mediator (acetylacetone, abbreviated as AA) into a hydrogel through the laccase-AA initiated polymerization. This self-initiated immobilization process avoided the forced conformational change of laccase in the passive embedding to pre-existing carriers. Resulting from the effective cooperation of laccase and AA, the Immo-LMS had the highest substrate conversion quantity to malachite green, followed by the sole immobilized laccase and the immobilized laccase with an external mediator. Besides the improved activity, the Immo-LMS showed enhanced stability. The good performance of the Immo-LMS suggests that the co-immobilization of laccase and mediator through the self-initiated one-pot process was a promising strategy for the immobilization of laccase, which is expected to be helpful to cut down the running cost as well as the potential toxicity that come from mediators in the practical application of laccase.
漆酶是一种绿色生物催化剂。它与分子氧一起作用，只产生水作为唯一的副产物。然而，由于游离漆酶的稳定性差/可重复使用性差，以及溶解介质可能带来的潜在二次污染，其实际应用远不尽如人意。为了解决基于漆酶的催化中的这些瓶颈问题，通过漆酶-AA 引发的聚合，将漆酶和一种介质（乙酰丙酮，简称 AA）同时固定在水凝胶中，制备了一种新型生物催化剂（Immo-LMS）。这种自引发的固定化过程避免了游离漆酶在被动嵌入现有载体时被迫发生构象变化。由于漆酶和 AA 的有效合作，Immo-LMS 对孔雀石绿的底物转化率最高，其次是单独固定的漆酶和外部介质固定的漆酶。除了提高活性外，Immo-LMS 还显示出增强的稳定性。Immo-LMS 的良好性能表明，通过自引发的一锅法同时固定漆酶和介质是固定化漆酶的一种有前途的策略，有望降低实际应用中漆酶的运行成本和潜在毒性。
