School of Life Sciences and Technology, Xinxiang Medical University, Jinsui Avenue 601, Xinxiang, Henan 453003, China.
School of Basic Medical Sciences, Xinxiang Medical University, Jinsui Avenue 601, Xinxiang, Henan 453003, China.
Biomed Res Int. 2018 Sep 24;2018:5471961. doi: 10.1155/2018/5471961. eCollection 2018.
The application of laccase-mediator-based catalysis is limited owing to the high cost of laccases and mediators and the potential toxicity of free mediators. Here, a novel biocatalyst (Im-LMS) was fabricated by immobilizing both laccase and a mediator (2,2'-azino-bis-[3-ethylbenzothiazoline]-6-sulfonic acid) on layered double hydroxide/alginate biohybrid beads. The catalytic activity of Im-LMS was evaluated for dye decolorization using malachite green. The decolorization yields of malachite green by Im-LMS and the free laccase-mediator system were 92% within 120 min and 90% within 90 min. Malachite green solution was detoxified completely after biodegradation by Im-LMS. Following eight reuse cycles of Im-LMS for dye treatment, a decolorization yield of 79% was obtained. The activity of Im-LMS was almost completely stable after being stored for 10 days. The recyclability and stability of Im-LMS will be helpful for reducing the running cost and potential toxicity associated with mediators to facilitate practical applications.
漆酶-介体催化的应用受到漆酶和介体成本高以及游离介体潜在毒性的限制。在这里，通过将漆酶和一种介体（2,2'-联氮双（3-乙基苯并噻唑啉-6-磺酸））固定在层状双氢氧化物/藻酸盐生物杂化珠上，制备了一种新型生物催化剂（Im-LMS）。使用孔雀石绿评估了 Im-LMS 对染料脱色的催化活性。Im-LMS 和游离漆酶-介体体系在 120 分钟内使孔雀石绿的脱色率分别达到 92%和 90%。Im-LMS 生物降解后，孔雀石绿溶液完全解毒。经过 8 次重复使用 Im-LMS 处理染料后，脱色率达到 79%。Im-LMS 储存 10 天后，其活性几乎完全稳定。Im-LMS 的可回收性和稳定性将有助于降低与介体相关的运行成本和潜在毒性，从而促进实际应用。
