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稳定固定在MIL-100(Fe)金属有机框架中的ABTS作为漆酶催化脱色的高效介质

Stable ABTS Immobilized in the MIL-100(Fe) Metal-Organic Framework as an Efficient Mediator for Laccase-Catalyzed Decolorization.

作者信息

Liu Youxun, Geng Yuanyuan, Yan Mingyang, Huang Juan

机构信息

School of Basic Medical Sciences, Xinxiang Medical University, Jinsui Avenue 601, Xinxiang 453003, Henan, China.

Henan Collaborative Innovation Center of Molecular Diagnostics and Laboratory Medicine, Jinsui Avenue 601, Xinxiang 453003, Henan, China.

出版信息

Molecules. 2017 Jun 2;22(6):920. doi: 10.3390/molecules22060920.

DOI:10.3390/molecules22060920
PMID:28574450
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6152645/
Abstract

The successful encapsulation of 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), a well-known laccase mediator, within a mesoporous metal-organic framework sample (i.e., MIL-100(Fe)) was achieved using a one-pot hydrothermal synthetic method. The as-prepared ABTS@MIL-100(Fe) was characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy, nitrogen sorption, and cyclic voltammetry (CV). Our ABTS@MIL-100(Fe)-based electrode exhibited an excellent electrochemical response, indicating that MIL-100(Fe) provides an appropriate microenvironment for the immobilization and electroactivity of ABTS molecules. ABTS@MIL-100(Fe) was then evaluated as an immobilized laccase mediator for dye removal using indigo carmine (IC) as a model dye. Through the application of laccase in combination with a free (ABTS) or immobilized (ABTS@MIL-100(Fe)) mediator, decolorization yields of 95% and 94%, respectively, were obtained for IC after 50 min. In addition, following seven reuse cycles of ABTS@MIL-100(Fe) for dye treatment, a decolorization yield of 74% was obtained. Dye decolorization occurred through the breakdown of the chromophoric group by the Laccase/ABTS@MIL-100(Fe) system, and a catalytic mechanism was proposed. We therefore expect that the stability, reusability, and validity of ABTS@MIL-100(Fe) as a laccase mediator potentially render it a promising tool for dye removal, in addition to reducing the high running costs and potential toxicity associated with synthetic mediators.

摘要

采用一锅水热合成法成功地将著名的漆酶介质2,2'-联氮双(3-乙基苯并噻唑啉-6-磺酸)(ABTS)包封在介孔金属有机骨架样品(即MIL-100(Fe))中。通过扫描电子显微镜(SEM)、X射线衍射(XRD)、傅里叶变换红外(FT-IR)光谱、氮吸附和循环伏安法(CV)对制备的ABTS@MIL-100(Fe)进行了表征。我们基于ABTS@MIL-100(Fe)的电极表现出优异的电化学响应，表明MIL-100(Fe)为ABTS分子的固定化和电活性提供了合适的微环境。然后，以靛蓝胭脂红(IC)为模型染料，评估ABTS@MIL-100(Fe)作为固定化漆酶介质用于染料去除的性能。通过将漆酶与游离(ABTS)或固定化(ABTS@MIL-100(Fe))介质联合使用，50分钟后IC的脱色率分别达到95%和94%。此外，ABTS@MIL-100(Fe)用于染料处理7个循环后，脱色率为74%。染料脱色是通过漆酶/ABTS@MIL-100(Fe)体系破坏发色基团实现的，并提出了催化机理。因此，我们预计ABTS@MIL-100(Fe)作为漆酶介质的稳定性、可重复使用性和有效性，除了降低与合成介质相关的高运行成本和潜在毒性外，还可能使其成为一种有前途的染料去除工具。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7074/6152645/eb9e5ab5f777/molecules-22-00920-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7074/6152645/dcab20df589f/molecules-22-00920-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7074/6152645/dcd8992772d1/molecules-22-00920-g002.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7074/6152645/37b2e83d3b2b/molecules-22-00920-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7074/6152645/d5a7f1c3420e/molecules-22-00920-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7074/6152645/d6c8a5b1d4f1/molecules-22-00920-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7074/6152645/417378363ac8/molecules-22-00920-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7074/6152645/2809a2195f53/molecules-22-00920-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7074/6152645/eb9e5ab5f777/molecules-22-00920-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7074/6152645/dcab20df589f/molecules-22-00920-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7074/6152645/dcd8992772d1/molecules-22-00920-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7074/6152645/7a4c0baedb39/molecules-22-00920-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7074/6152645/37b2e83d3b2b/molecules-22-00920-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7074/6152645/d5a7f1c3420e/molecules-22-00920-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7074/6152645/d6c8a5b1d4f1/molecules-22-00920-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7074/6152645/417378363ac8/molecules-22-00920-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7074/6152645/2809a2195f53/molecules-22-00920-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7074/6152645/eb9e5ab5f777/molecules-22-00920-g009.jpg

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