Li Xue, Zhou Chi, Li Ming, Zhang Qingzhuang, Su Lei, Li Xin
National Human Diseases Animal Model Resource Center, Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS) & Peking Union Medical College (PUMC), Beijing 100021, China.
Hunan Institute of Microbiology, Changsha 410009, China.
Life (Basel). 2025 Feb 24;15(3):354. doi: 10.3390/life15030354.
In an investigation exploring endophytic microbiota from agricultural crops, an aerobic, non-motile, Gram-negative, coccobacillus-shaped bacterial isolate, designated as strain NGMCC 1.201697, was isolated from maize roots in Hunan Province, China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain NGMCC 1.201697 belonged to the genus , showing the highest sequence similarity to CPCC 101403 (99.86%). The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) were 98.57% and 87.90% between the novel isolate and its closest phylogenetic relative. However, phenotypic characterization further differentiated the isolate from CPCC 101403. The isolate showed enhanced environmental tolerance adaptability (growth in 0-8% NaCl and 4-37 °C), unique enzymatic activities (esterase C4, β-glucosidase, L-proline arylamidase, and β-galactosidase), and expanded metabolic capabilities (D-mannitol, D-cellobiose, saccharose, and so on). The major polar lipids consisted of diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidylglycerol (PG), two unidentified glycolipids (GLs) and four unidentified phospholipids (PLs). The predominant respiratory quinone was ubiquinone-10, and the major fatty acid was summed feature 8 (C18:1 ω7c, 69.42%). The DNA G + C content was 64.49 mol%. Based on results of these analyses, strain NGMCC 1.201697 represents a novel subspecies of , for which the name subsp. drimophilus subsp. nov. is proposed. The type-strain is NGMCC 1.201697 (=CGMCC 1.61958 =JCM 37104).
在一项对农作物内生微生物群的研究中，从中国湖南省的玉米根部分离出一株需氧、不运动、革兰氏阴性、球杆菌状的细菌菌株，命名为菌株NGMCC 1.201697。基于16S rRNA基因序列的系统发育分析表明，菌株NGMCC 1.201697属于 属，与CPCC 101403的序列相似性最高（99.86%）。该新分离菌株与其最接近的系统发育亲缘关系菌株之间的平均核苷酸同一性（ANI）和数字DNA-DNA杂交（dDDH）分别为98.57%和87.90%。然而，表型特征进一步将该分离菌株与CPCC 101403区分开来。该分离菌株表现出增强的环境耐受性适应性（在0-8% NaCl和4-37°C下生长）、独特的酶活性（酯酶C4、β-葡萄糖苷酶、L-脯氨酸芳基酰胺酶和β-半乳糖苷酶）以及扩展的代谢能力（D-甘露醇、D-纤维二糖、蔗糖等）。主要极性脂质由双磷脂酰甘油（DPG）、磷脂酰乙醇胺（PE）、磷脂酰胆碱（PC）、磷脂酰甘油（PG）、两种未鉴定的糖脂（GLs）和四种未鉴定的磷脂（PLs）组成。主要呼吸醌为泛醌-10，主要脂肪酸为总和特征8（C18:1 ω7c，69.42%）。DNA G + C含量为64.49 mol%。基于这些分析结果，菌株NGMCC 1.201697代表 的一个新亚种，为此提出名称 亚种drimophilus亚种。模式菌株为NGMCC 1.201697（=CGMCC 1.61958 =JCM 37104）。
