Kamble Prasad G, Pereira Maria J, Sidibeh Cherno O, Amini Sam, Sundbom Magnus, Börjesson Joey Lau, Eriksson Jan W
Department of Medical Sciences, Uppsala University, Uppsala, Sweden.
Department of Surgery, Uppsala University, Uppsala, Sweden.
Mol Cell Endocrinol. 2016 May 15;427:124-32. doi: 10.1016/j.mce.2016.03.011. Epub 2016 Mar 10.
The adipokine lipocalin 2 is linked to obesity and metabolic disorders. However, its role in human adipose tissue glucose and lipid metabolism is not explored. Here we show that the synthetic glucocorticoid dexamethasone dose-dependently increased lipocalin 2 gene expression in subcutaneous and omental adipose tissue from pre-menopausal females, while it had no effect in post-menopausal females or in males. Subcutaneous adipose tissue from both genders treated with recombinant human lipocalin 2 showed a reduction in protein levels of GLUT1 and GLUT4 and in glucose uptake in isolated adipocytes. In subcutaneous adipose tissue, lipocalin 2 increased IL-6 gene expression whereas expression of PPARγ and adiponectin was reduced. Our findings suggest that lipocalin 2 can contribute to insulin resistance in human adipose tissue. In pre-menopausal females, it may partly mediate adverse metabolic effects exerted by glucocorticoid excess.
脂肪因子2与肥胖和代谢紊乱有关。然而，其在人体脂肪组织葡萄糖和脂质代谢中的作用尚未得到研究。在此，我们表明，合成糖皮质激素地塞米松可剂量依赖性地增加绝经前女性皮下和网膜脂肪组织中2基因的表达，而对绝经后女性或男性则无影响。用重组人2处理的两性皮下脂肪组织显示，分离的脂肪细胞中葡萄糖转运蛋白1（GLUT1）和葡萄糖转运蛋白4（GLUT4）的蛋白水平以及葡萄糖摄取均降低。在皮下脂肪组织中，2增加白细胞介素6（IL-6）基因的表达，而过氧化物酶体增殖物激活受体γ（PPARγ）和脂联素的表达则降低。我们的研究结果表明，2可能导致人体脂肪组织中的胰岛素抵抗。在绝经前女性中，它可能部分介导糖皮质激素过量所产生的不良代谢效应。
