Liu Yan, Liu Sheng, Wu Hong-Hui, Zhang Xiang
Department of Pediatrics, Xianning Central Hospital, Xianning, Hubei 437100, China.
Zhongguo Dang Dai Er Ke Za Zhi. 2016 Mar;18(3):243-6. doi: 10.7499/j.issn.1008-8830.2016.03.011.
To investigate the association of two single-nucleotide polymorphisms (SNPs) in IL1R1 gene (rs1558641 and rs949963) with the susceptibility to asthma in children from Central China.
A case-control study was performed in the asthma group and the control group, consisting of 208 children with asthma and 223 normal children from Central China, respectively. The genotypes of two SNPs in IL1R1 gene, rs1558641 and rs949963, were identified using polymerase chain reaction-restriction fragment length polymorphism. The serum level of IL1R1 was determined by enzyme-linked immunosorbent assay.
There were no significant differences in genotype and allele frequencies of rs1558641 between the asthma and control groups. In terms of rs949963, the frequencies of GG genotype and alleles were significantly higher in the asthma group than in the control group (P<0.05). The asthma group had a significantly higher serum level of IL1R1 than the control group (P=0.011). Moreover, the serum level of IL1R1 was significantly higher in patients with GG genotype than in those with AA or AG genotype for rs949963 (P=0.028).
IL1R1 SNP rs949963 is associated with the susceptibility to asthma in children from Central China and may increase the serum expression of IL1R1.
探讨白细胞介素1受体1（IL1R1）基因中的两个单核苷酸多态性（SNP）（rs1558641和rs949963）与中国中部地区儿童哮喘易感性的关系。
进行病例对照研究，哮喘组和对照组分别由来自中国中部地区的208例哮喘儿童和223例正常儿童组成。采用聚合酶链反应-限制性片段长度多态性方法鉴定IL1R1基因中两个SNP（rs1558641和rs949963）的基因型。通过酶联免疫吸附测定法测定血清IL1R1水平。
哮喘组和对照组之间rs1558641的基因型和等位基因频率无显著差异。就rs949963而言，哮喘组中GG基因型和等位基因的频率显著高于对照组（P<0.05）。哮喘组的血清IL1R1水平显著高于对照组（P=0.011）。此外，rs949963的GG基因型患者的血清IL1R1水平显著高于AA或AG基因型患者（P=0.028）。
IL1R1 SNP rs949963与中国中部地区儿童哮喘易感性相关，可能增加IL1R1的血清表达。
