快速干涉二次谐波产生显微镜术
Fast interferometric second harmonic generation microscopy.
作者信息
Bancelin Stéphane, Couture Charles-André, Légaré Katherine, Pinsard Maxime, Rivard Maxime, Brown Cameron, Légaré François
机构信息
Institut National de la Recherche Scientifique, Centre Énergie Matériaux Télécommunications (INRS-EMT); 1650 Boul. Lionel-Boulet, Varennes (QC), J3X 1S2, Canada.
University of Oxford, Botnar Research Center, NDORMS, Windmill Road, Oxford, OX3 7HE, UK.
出版信息
Biomed Opt Express. 2016 Jan 8;7(2):399-408. doi: 10.1364/BOE.7.000399. eCollection 2016 Feb 1.
Abstract
We report the implementation of fast Interferometric Second Harmonic Generation (I-SHG) microscopy to study the polarity of non-centrosymmetric structures in biological tissues. Using a sample quartz plate, we calibrate the spatially varying phase shift introduced by the laser scanning system. Compensating this phase shift allows us to retrieve the correct phase distribution in periodically poled lithium niobate, used as a model sample. Finally, we used fast interferometric second harmonic generation microscopy to acquire phase images in tendon. Our results show that the method exposed here, using a laser scanning system, allows to recover the polarity of collagen fibrils, similarly to standard I-SHG (using a sample scanning system), but with an imaging time about 40 times shorter.
摘要
我们报告了快速干涉二次谐波产生（I-SHG）显微镜的应用，以研究生物组织中非中心对称结构的极性。使用一块石英样品板，我们校准了激光扫描系统引入的空间变化相移。补偿该相移使我们能够在用作模型样品的周期性极化铌酸锂中检索正确的相位分布。最后，我们使用快速干涉二次谐波产生显微镜获取肌腱的相位图像。我们的结果表明，这里展示的使用激光扫描系统的方法能够恢复胶原纤维的极性，类似于标准I-SHG（使用样品扫描系统），但成像时间缩短了约40倍。
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