利用干涉二次谐波产生显微镜成像肌球蛋白丝的双极性。
Imaging the bipolarity of myosin filaments with Interferometric Second Harmonic Generation microscopy.
作者信息
Rivard Maxime, Couture Charles-André, Miri Amir K, Laliberté Mathieu, Bertrand-Grenier Antony, Mongeau Luc, Légaré François
机构信息
Institut National de la Recherche Scientifique, Centre Énergie Matériaux Télécommunications, 1650 Boulevard Lionel-Boulet, Varennes, Quebec, J3X 1S2, Canada.
出版信息
Biomed Opt Express. 2013 Sep 9;4(10):2078-86. doi: 10.1364/BOE.4.002078. eCollection 2013.
Abstract
We report that combining interferometry with Second Harmonic Generation (SHG) microscopy provides valuable information about the relative orientation of noncentrosymmetric structures composing tissues. This is confirmed through the imaging of rat medial gastrocnemius muscle. The inteferometric Second Harmonic Generation (ISHG) images reveal that each side of the myosin filaments composing the A band of the sarcomere generates π phase shifted SHG signal which implies that the myosin proteins at each end of the filaments are oriented in opposite directions. This highlights the bipolar structural organization of the myosin filaments and shows that muscles can be considered as a periodically poled biological structure.
摘要
我们报告称，将干涉测量法与二次谐波产生（SHG）显微镜相结合，可为构成组织的非中心对称结构的相对取向提供有价值的信息。这一点通过对大鼠腓肠肌内侧的成像得到了证实。干涉二次谐波产生（ISHG）图像显示，构成肌节A带的肌球蛋白丝的每一侧都会产生π相移的SHG信号，这意味着丝两端的肌球蛋白蛋白取向相反。这突出了肌球蛋白丝的双极结构组织，并表明肌肉可被视为一种周期性极化的生物结构。
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