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用于研究细胞内脂质储存和动态变化的显微镜工具。

Microscopy tools for the investigation of intracellular lipid storage and dynamics.

作者信息

Daemen Sabine, van Zandvoort Marc A M J, Parekh Sapun H, Hesselink Matthijs K C

机构信息

Department of Human Movement Sciences and Human Biology, NUTRIM School for Nutrition and Translational Research in Metabolism, Maastricht University, Maastricht, The Netherlands.

Department of Genetics and Molecular Cell Biology, CARIM School for Cardiovascular Diseases, Maastricht University, Maastricht, The Netherlands; Institute for Molecular Cardiovascular Research (IMCAR), RWTH Aachen University, Aachen, Germany.

出版信息

Mol Metab. 2015 Dec 31;5(3):153-163. doi: 10.1016/j.molmet.2015.12.005. eCollection 2016 Mar.

DOI:10.1016/j.molmet.2015.12.005
PMID:26977387
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4770264/
Abstract

BACKGROUND

Excess storage of lipids in ectopic tissues, such as skeletal muscle, liver, and heart, seems to associate closely with metabolic abnormalities and cardiac disease. Intracellular lipid storage occurs in lipid droplets, which have gained attention as active organelles in cellular metabolism. Recent developments in high-resolution microscopy and microscopic spectroscopy have opened up new avenues to examine the physiology and biochemistry of intracellular lipids.

SCOPE OF REVIEW

The aim of this review is to give an overview of recent technical advances in microscopy, and its application for the visualization, identification, and quantification of intracellular lipids, with special focus to lipid droplets. In addition, we attempt to summarize the probes currently available for the visualization of lipids.

MAJOR CONCLUSIONS

The continuous development of lipid probes in combination with the rapid development of microscopic techniques can provide new insights in the role and dynamics of intracellular lipids. Moreover, in situ identification of intracellular lipids is now possible and promises to add a new dimensionality to analysis of lipid biochemistry, and its relation to (patho)physiology.

摘要

背景

脂质在异位组织（如骨骼肌、肝脏和心脏）中的过度储存似乎与代谢异常和心脏疾病密切相关。细胞内脂质储存在脂滴中，脂滴作为细胞代谢中的活跃细胞器已受到关注。高分辨率显微镜和显微光谱学的最新进展为研究细胞内脂质的生理学和生物化学开辟了新途径。

综述范围

本综述的目的是概述显微镜技术的最新技术进展及其在细胞内脂质可视化、鉴定和定量方面的应用，特别关注脂滴。此外，我们试图总结目前可用于脂质可视化的探针。

主要结论

脂质探针的不断发展与显微技术的快速发展相结合，可以为细胞内脂质的作用和动态提供新的见解。此外，现在可以对细胞内脂质进行原位鉴定，并有望为脂质生物化学及其与（病理）生理学关系的分析增添新的维度。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c653/4770264/e3d678491ba9/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c653/4770264/52bd9b650e67/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c653/4770264/881431d3ce3c/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c653/4770264/3c3589647db7/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c653/4770264/f4fbdee46805/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c653/4770264/efbb576fb3c2/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c653/4770264/e3d678491ba9/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c653/4770264/52bd9b650e67/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c653/4770264/881431d3ce3c/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c653/4770264/3c3589647db7/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c653/4770264/f4fbdee46805/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c653/4770264/efbb576fb3c2/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c653/4770264/e3d678491ba9/gr6.jpg

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