Srivastava Anup, Shinn Amanda S, Lam TuKiet T, Lee Patty J, Mannam Praveen
Pulmonary, Critical Care and Sleep Medicine, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06520-8057, USA.
MS & Proteomics Resource at Yale University, WM Keck Foundation Biotechnology Resource Laboratory, Department of Molecular Biophysics and Biochemistry, New Haven, CT 06520-8057, USA.
Data Brief. 2016 Mar 2;7:418-22. doi: 10.1016/j.dib.2016.02.034. eCollection 2016 Jun.
This data article reports changes in the phospho and total proteome of MKK3 knock out (MKK3(-) (/) (-)) mouse embryonic fibroblasts (MEFs). The dataset generated highlights the changes at protein level which can be helpful for understanding targets of the MAP kinase signaling pathway. Data was collected after TiO2-based phosphopeptide enrichment of whole cell lysate at baseline condition with bottom-up SILAC-based LC MS/MS quantitative mass spectrometry. We report all the proteins and peptides identified and quantified in MKK3(-/-) and WT MEFs. The altered pathways in MKK3(-/-) MEFs were analyzed by Database for Annotation, Visualization and Integrated Discovery (DAVID, v6.7) and Ingenuity Pathway Analysis (IPA) and are presented as a table and graph, respectively. The data reported here is related to the published work [1]. All the associated mass spectrometry data has been deposited in the Yale Protein Expression Database (YPED) with the web-link to the data: http://yped.med.yale.edu/repository/ViewSeriesMenu.do;jsessionid=6A5CB07543D8B529FAE8C3FCFE29471D?series_id=5044&series_name=MMK3+Deletion+in+MEFs.
本数据文章报告了MKK3基因敲除（MKK3(-)/(-)）小鼠胚胎成纤维细胞（MEF）的磷酸化蛋白质组和总蛋白质组的变化。所生成的数据集突出了蛋白质水平的变化，这有助于理解丝裂原活化蛋白激酶信号通路的靶点。在基线条件下，通过基于二氧化钛的磷酸肽富集全细胞裂解物，并采用自下而上的基于稳定同位素标记氨基酸的细胞培养（SILAC）液相色谱-质谱联用（LC MS/MS）定量质谱法收集数据。我们报告了在MKK3(-/-)和野生型（WT）MEF中鉴定和定量的所有蛋白质和肽段。通过注释、可视化和综合发现数据库（DAVID，v6.7）和 Ingenuity 通路分析（IPA）分别对MKK3(-/-) MEF中改变的通路进行了分析，并以表格和图表形式呈现。此处报告的数据与已发表的工作[1]相关。所有相关的质谱数据已存入耶鲁蛋白质表达数据库（YPED），数据的网络链接为：http://yped.med.yale.edu/repository/ViewSeriesMenu.do;jsessionid=6A5CB07543D8B529FAE8C3FCFE29471D?series_id=5044&series_name=MMK3+Deletion+in+MEFs 。
