Departamento de Patologia, Faculdade de Medicina Veterinária, Universidade de São Paulo, São Paulo, SP, Brazil; Facultad de Ciencias Agropecuarias y Recursos Naturales, Programa de Medicina Veterinaria y Zootecnia, Universidad de los Llanos, Villavicencio, Colombia.
Universidade de Santo Amaro, São Paulo, SP, Brazil.
Life Sci. 2016 Apr 15;151:281-287. doi: 10.1016/j.lfs.2016.03.027. Epub 2016 Mar 12.
To evaluate the influence of lactation on lung immune function during allergic inflammation.
Female rats, 60-90days old, were divided into three groups: no lung allergy virgins (N group), ovalbumin (OVA)-immunized and sensitized virgins (V group), and OVA-immunized and sensitized lactating females (L group). On gestation day (GD) 10, all animals in L group received a subcutaneous injection of 0.1mg·kg(-1) OVA plus aluminum hydroxide. On GD17, the L group received a subcutaneous booster injection of 10μg OVA plus 10mg aluminum hydroxide. After 7days, an inhalatory challenge with 1% OVA was given in 15min sessions for 3 consecutive days. Animals from the V group received the same treatment, meaning both tests and time intervals between OVA treatment and inhalatory challenge were the same as in the L group. Twenty-four hours after the last inhalation session, the animals were euthanized, and the following tests were performed: total and differential bronchoalveolar lavage (BAL) and femoral marrow lavage (FML) leukocyte counts, quantification of tumor necrosis factor α (TNF-α) and interferon γ (IFN-γ) levels in BAL fluid, and quantification of plasma corticosterone and catecholamine levels.
The L group presented lower BAL total leukocyte counts and decreases in the number of eosinophils and macrophages compared with the V group. They also expressed higher BAL IFN-γ and lower plasma corticosterone levels. Plasma norepinephrine levels were higher in the L group than in the N and V groups.
Lactating female rats presented less intense allergic lung inflammation. Our findings suggest that lactation may protect females from asthmatic crises.
评估哺乳期对变应性炎症期间肺免疫功能的影响。
60-90 日龄雌性大鼠分为三组：无肺过敏处女鼠（N 组）、卵清蛋白（OVA）免疫致敏处女鼠（V 组）和 OVA 免疫致敏哺乳期雌性鼠（L 组）。在妊娠第 10 天，L 组所有动物均接受 0.1mg·kg(-1)OVA 加氢氧化铝皮下注射。在妊娠第 17 天，L 组接受 10μg OVA 加 10mg 氢氧化铝的皮下加强注射。7 天后，以 15min 为间隔进行为期 3 天的 1%OVA 吸入性挑战。V 组接受相同的治疗，即 OVA 治疗和吸入性挑战之间的测试和时间间隔均与 L 组相同。最后一次吸入后 24 小时，处死动物，进行以下测试：支气管肺泡灌洗（BAL）和股骨骨髓灌洗（FML）总白细胞计数和分类计数、BAL 液中肿瘤坏死因子 α（TNF-α）和干扰素 γ（IFN-γ）水平的定量以及血浆皮质酮和儿茶酚胺水平的定量。
L 组 BAL 总白细胞计数较低，嗜酸性粒细胞和巨噬细胞数量减少，BAL IFN-γ 水平较高，血浆皮质酮水平较低。与 N 组和 V 组相比，L 组血浆去甲肾上腺素水平较高。
哺乳期雌性大鼠表现出较轻的变应性肺炎症。我们的发现表明哺乳期可能保护女性免受哮喘发作的影响。
