Ochoa-Amaya Julieta E, Hamasato Eduardo K, Tobaruela Carla N, Queiroz-Hazarbassanov Nicolle, Anselmo Franci Janete A, Palermo-Neto João, Greiffo Flavia R, de Britto Auriléia Aparecida, Vieira Rodolfo Paula, Ligeiro de Oliveira Ana P, Massoco Cristina de O, Felicio Luciano F
Departamento de Patologia da Faculdade de Medicina Veterináriae Zootecnia da Universidade de São Paulo, São Paulo, SP, Brazil; Facultad de Ciencias Agropecuarias y Recursos Naturales, Programa de Medicina Veterinaria y Zootecnia, Universidad de los Llanos, Villavicencio, Colombia.
Departamento de Patologia da Faculdade de Medicina Veterináriae Zootecnia da Universidade de São Paulo, São Paulo, SP, Brazil.
Life Sci. 2015 Dec 1;142:66-75. doi: 10.1016/j.lfs.2015.10.016. Epub 2015 Oct 21.
Prolactin is a major immunomodulator. The present study evaluated the effects of short-term hyperprolactinemia induced by domperidone before ovalbumin antigenic challenge on the lung's allergic inflammatory response.
To induce hyperprolactinemia, domperidone was injected in rats at a dose of 5.1mg·kg(-1) per day, i.p., for 5days from 10th to 14th day after OVA immunization. Total and differential leukocyte counts from bronchoalveolar lavage (BAL), femoral marrow lavage (FML), and blood were analyzed. The percentages of mucus and collagen production were evaluated. Levels of corticosterone and prolactin in serum, interleukin-4 (IL-4), IL-6, IL-10, tumor necrosis factor α (TNF-α) in lung explants supernatants were measured and interferon gamma (IFN-γ) in bronchiolar lavage cells suspensions (BAL) was measured.
The rats that were subjected to short-term hyperprolactinemia exhibited a decrease in leukocyte counts in bronchoalveolar lavage, cellularity decrease in femoral marrow lavage fluid, a lower percentage of mucus, and an increase in lung IL-4, IL-6, IL-10, TNF-α and IFN-γ expression.
Hyperprolactinemia induced before antigenic challenge decreased allergic lung inflammation. These data suggest that prolactin may play a role in the pathophysiology of asthma. The present study demonstrates a prospective beneficial side effect of domperidone for asthmatic patients.
催乳素是一种主要的免疫调节剂。本研究评估了在卵清蛋白抗原激发前由多潘立酮诱导的短期高催乳素血症对肺部过敏性炎症反应的影响。
为诱导高催乳素血症，从卵清蛋白免疫后第10天至第14天，以每天5.1mg·kg(-1)的剂量腹腔注射多潘立酮给大鼠，持续5天。分析支气管肺泡灌洗（BAL）、股骨骨髓灌洗（FML）和血液中的白细胞总数及分类计数。评估黏液和胶原蛋白产生的百分比。测量血清中皮质酮和催乳素的水平、肺外植体上清液中的白细胞介素-4（IL-4）、IL-6、IL-10、肿瘤坏死因子α（TNF-α），并测量细支气管灌洗细胞悬液（BAL）中的干扰素γ（IFN-γ）。
经历短期高催乳素血症的大鼠支气管肺泡灌洗中的白细胞计数减少、股骨骨髓灌洗液中的细胞数量减少、黏液百分比降低，且肺中IL-4、IL-6、IL-10、TNF-α和IFN-γ表达增加。
抗原激发前诱导的高催乳素血症减轻了过敏性肺部炎症。这些数据表明催乳素可能在哮喘的病理生理学中起作用。本研究证明了多潘立酮对哮喘患者的一种潜在有益副作用。
