Ochoa-Amaya Julieta Esperanza, Paula Ligeiro de Oliveira Ana, Luciano Freitas Felicio, Bernardi Maria Martha
University of the Llanos, Faculty of Agricultural Sciences and Natural Resources, School of Animal Sciences, Colombia.
Department of Pathology, School of Veterinary Medicine, University of São Paulo, São Paulo, Brazil.
Brain Behav Immun Health. 2024 Jul 20;40:100822. doi: 10.1016/j.bbih.2024.100822. eCollection 2024 Oct.
Asthma is characterized by pulmonary cell infiltration and hyper-responsiveness of the airways. Short-term stress reduces airway inflammation. Thus, in the present study, we examined the effects of short-term stress induced by repeated treatment with saline injections on the pulmonary allergic inflammatory response in rats.
Adult male rats were divided into three groups: Naïve group (non-sensitized, challenged, or treated rats), Control group (rats sensitized with ovalbumin (OVA) to induce lung inflammation), and Saline group (rats treated for five days with saline before OVA sensitization). Inhalation challenges were performed one week after the booster with aerosolized OVA. On day 18, the effect of saline injections on total and differential leukocytes in bronchoalveolar lavage (BAL), femoral marrow lavage (FML), and blood was evaluated. The percentage of mucus, serum corticosterone, collagen, cytokines in lung explants, and norepinephrine levels were also measured.
OVA sensitization increased the circulating leukocytes and their migration to the lung, decreasing the bone marrow leukocytes. The repeated saline injections prevented this migration by decreasing the number of leukocytes in BAL and blood in the control group. Cytokine Interleukin-4 (IL-4) was higher in the control group than in the naive and saline groups; cytokines Interleukin-6 (IL-6), Interleukin-10 (IL-10), and tumor necrosis factor-alpha (TNFα) were higher in the control and saline groups than in the naïve group; Interferon gamma (IFNγ) was higher in the saline group than in the naive and control groups; norepinephrine increased in animals sensitized with OVA and was higher only in the saline group relative to the naïve group.
These results suggest that short-term stress could contribute to the anti-allergic airway inflammation effects of a given treatment.
哮喘的特征是肺细胞浸润和气道高反应性。短期应激可减轻气道炎症。因此，在本研究中，我们检测了通过反复注射生理盐水诱导的短期应激对大鼠肺部过敏性炎症反应的影响。
成年雄性大鼠分为三组：未处理组（未致敏、未激发或未处理的大鼠）、对照组（用卵清蛋白（OVA）致敏以诱导肺部炎症的大鼠）和生理盐水组（在OVA致敏前用生理盐水处理五天的大鼠）。在加强免疫一周后，用雾化OVA进行吸入激发。在第18天，评估生理盐水注射对支气管肺泡灌洗（BAL）、股骨髓灌洗（FML）和血液中白细胞总数及分类的影响。还测量了肺组织中黏液百分比、血清皮质酮、胶原蛋白、细胞因子以及去甲肾上腺素水平。
OVA致敏增加了循环白细胞及其向肺部的迁移，减少了骨髓白细胞。反复注射生理盐水通过减少对照组BAL和血液中的白细胞数量来阻止这种迁移。对照组中细胞因子白细胞介素-4（IL-4）高于未处理组和生理盐水组；细胞因子白细胞介素-6（IL-6）、白细胞介素-10（IL-10）和肿瘤坏死因子-α（TNFα）在对照组和生理盐水组中高于未处理组；干扰素-γ（IFNγ）在生理盐水组中高于未处理组和对照组；OVA致敏动物的去甲肾上腺素增加，且仅生理盐水组相对于未处理组更高。
这些结果表明短期应激可能有助于某种特定治疗的抗过敏气道炎症效应。
