Gutiérrez P, Mesa H Jaramillo, Marín Montoya M
Acta Virol. 2016 Mar;60(1):49-54. doi: 10.4149/av_2016_01_49.
Deep sequencing analysis of the transcriptome of a Solanum phureja cv. Criolla Colombia plant with symptoms typical of a virus disease revealed an infection with potato virus V (PVV). The PVV-phureja genome comprises 9904 nt, exhibits 83% nucleotide identity with currently fully sequenced PVV isolates and contains one large ORF that codes for a polyprotein of 3065 residues flanked by 5' and 3' UTR of 217 and 448 nt, respectively. Phylogenetic analysis of the PVV-phureja polyprotein indicates that it is divergent with respect to most PVV isolates. This is the first complete PVV genome of an isolate infecting a host different to S. tuberosum and, to this date, the only one from the South American Andes.
对一株具有典型病毒病症状的哥伦比亚克里奥亚亚种马铃薯（Solanum phureja cv. Criolla Colombia）植株的转录组进行深度测序分析，结果显示该植株感染了马铃薯病毒V（PVV）。PVV-克里奥亚亚种的基因组由9904个核苷酸组成，与目前已完全测序的PVV分离株具有83%的核苷酸同一性，并且包含一个大的开放阅读框，该阅读框编码一个由3065个氨基酸残基组成的多聚蛋白，其两侧分别为217个核苷酸和448个核苷酸的5'和3'非翻译区。对PVV-克里奥亚亚种多聚蛋白的系统发育分析表明，它与大多数PVV分离株不同。这是感染不同于马铃薯（S. tuberosum）的宿主的分离株的首个完整PVV基因组，并且截至目前，是来自南美安第斯山脉的唯一一株。
