一种感染葫芦科植物的新型马铃薯Y病毒的鉴定与特性分析
Identification and characterization of a new potyvirus infecting cucurbits.
作者信息
Perotto M C, Pozzi E A, Celli M G, Luciani C E, Mitidieri M S, Conci V C
机构信息
Instituto de Patología Vegetal (IPAVE-CIAP-INTA), Camino 60 cuadras km 5,5, Córdoba, X5020ICA, Argentina.
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Camino 60 cuadras km 5,5, Córdoba, X5020ICA, Argentina.
出版信息
Arch Virol. 2018 Mar;163(3):719-724. doi: 10.1007/s00705-017-3660-2. Epub 2017 Dec 1.
A new potyvirus, tentatively named cucurbit vein banding virus (CVBV), was identified in crops of cucurbits in San Pedro (Buenos Aires, Argentina). The complete genome sequences of two isolates of CVBV were obtained by next-generation sequencing (Illumina). The genomic RNA consisted of 9968 and 9813 nucleotides, respectively, and displayed typical potyvirus organization. The percentage identity for these two genome sequences, using BLASTn, was 77% to sweet potato virus c and 73% to tomato necrotic stunt virus. BLASTx analysis of the complete polyprotein showed that the most closely related virus is plum pox virus, with 48% amino acid sequence identity for both isolates. Sequence comparisons and phylogenetic analyses indicate that CVBV belongs to a previously undescribed species in genus Potyvirus.
在阿根廷布宜诺斯艾利斯省圣佩德罗的葫芦科作物中鉴定出一种新的马铃薯Y病毒，暂命名为葫芦科脉带病毒（CVBV）。通过下一代测序（Illumina）获得了CVBV两个分离株的完整基因组序列。基因组RNA分别由9968和9813个核苷酸组成，呈现典型的马铃薯Y病毒结构。使用BLASTn对这两个基因组序列进行比对，与甘薯病毒c的序列一致性为77%，与番茄坏死矮化病毒的序列一致性为73%。对完整多聚蛋白的BLASTx分析表明，亲缘关系最密切的病毒是李痘病毒，两个分离株的氨基酸序列一致性均为48%。序列比较和系统发育分析表明，CVBV属于马铃薯Y病毒属中一个以前未描述过的种。
Zotero 插件