Żerańska Justyna, Pasikowska Monika, Szczepanik Barbara, Mlosek Krzysztof, Malinowska Sylwia, Dębowska Renata M, Eris Irena
Dr Irena Eris Cosmetic Laboratories, Dr Irena Eris Centre for Science and Research, Warsaw, Poland; Department of Cosmetic Raw Materials Chemistry, Medical University of Lodz, Lodz, Poland.
Department of Cosmetic Raw Materials Chemistry, Medical University of Lodz, Lodz, Poland.
Postepy Dermatol Alergol. 2016 Feb;33(1):28-36. doi: 10.5114/pdia.2014.44024. Epub 2016 Feb 29.
Fibroblast growth factor 1 (FGF-1) is a powerful mitogen involved in the stimulation of DNA synthesis and the proliferation of a wide variety of cell types. Fibroblast growth factor 1 was genetically modified to improve its thermal stability and resistance to protease degradation without losing its biological activity.
To study the impact of Q40P/S47I/H93G rFGF-1 on skin cells, its penetration through the skin and the evaluation of the rFGF-1-cosmetic product properties.
In vitro studies included the examination of primary fibroblast and keratinocyte viability after the incubation with rFGF-1. The penetration abilities of rFGF-1 in various formulations and carrier systems were examined ex vivo by the Raman spectroscopy. In vivo studies - HF Ultrasound and 3D Imaging System - were used to evaluate the anti-aging properties of creams containing rFGF-1.
In vitro studies demonstrated that rFGF-1 strongly enhanced the viability of the treated cells. The Raman Spectroscopy analysis indicated that rFGF-1 encapsulated in lipid spheres penetrate through the stratum corneum to the depth of 60 µm, and added to the o/w formulation - could penetrate to a depth of 90 µm. The results obtained from Primos revealed the reduction of the volume and the depth of the wrinkles. Changes in the skin structure in the analyzed areas were evaluated by HF Ultrasonography.
Recombinant FGF-1 strongly stimulated fibroblast and keratinocyte proliferation. However, the transition of this protein through the SC required an appropriate carrier system - lipid spheres. All tests - in vitro, ex vivo and in vivo - have proved that rFGF-1 is a substance with a potentially wide spectrum of use.
成纤维细胞生长因子1（FGF-1）是一种强大的促有丝分裂原，参与刺激DNA合成以及多种细胞类型的增殖。对成纤维细胞生长因子1进行基因改造，以提高其热稳定性和对蛋白酶降解的抗性，同时不丧失其生物活性。
研究Q40P/S47I/H93G重组人成纤维细胞生长因子1（rFGF-1）对皮肤细胞的影响、其透过皮肤的情况以及对含rFGF-1化妆品产品特性的评估。
体外研究包括在与rFGF-1孵育后检测原代成纤维细胞和角质形成细胞的活力。通过拉曼光谱体外检测rFGF-1在各种制剂和载体系统中的渗透能力。体内研究——高频超声和三维成像系统——用于评估含rFGF-1乳膏的抗老化特性。
体外研究表明，rFGF-1强烈增强了处理后细胞的活力。拉曼光谱分析表明，包裹在脂质球中的rFGF-1可穿透角质层至60μm深度，添加到水包油制剂中——可穿透至90μm深度。从Primos获得的结果显示皱纹的体积和深度减小。通过高频超声评估分析区域皮肤结构的变化。
重组FGF-1强烈刺激成纤维细胞和角质形成细胞增殖。然而，这种蛋白质透过角质层需要合适的载体系统——脂质球。所有体外、离体和体内试验均证明rFGF-1是一种具有潜在广泛用途的物质。
