Bazrgar Masood, Gourabi Hamid, Eftekhari-Yazdi Poopak, Vazirinasab Hamed, Fakhri Mostafa, Hassani Fatemeh, Chehrazi Mohamad, Valojerdi Mojtaba Rezazadeh
Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran; Department of Developmental Biology, University of Science and Culture, Tehran, Iran.
Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
Int J Fertil Steril. 2016 Jan-Mar;9(4):563-73. doi: 10.22074/ijfs.2015.4617. Epub 2015 Dec 23.
A decrease in aneuploidy rate following a prolonged co-culture of human blastocysts has been reported. As co-culture is not routinely used in assisted reproductive technology, the present study aimed to evaluate the effect of the prolonged single culture on the rate of diploid cells in human embryos with aneuploidies.
In this cohort study, we used fluorescence in situ hybridi- zation (FISH) to reanalyze surplus blastocysts undergoing preimplantation genetic diagnosis (PGD) on day 3 postfertilization. They were randomly studied on days 6 or 7 following fertilization.
Of the 30 analyzed blastocysts, mosaicism was observed in 26(86.6%), while 2(6.7%) were diploid, and 2(6.7%) were triploid. Of those with mosaicism, 23(88.5%) were determined to be diploid-aneuploid and 3(11.5%) were aneuploid mosaic. The total frequency of embryos with more than 50% diploid cells was 33.3% that was lower on day 7 in comparison with the related value on day 6 (P<0.05); however, there were no differences when the embryos were classified according to maternal age, blastocyst developmental stage, total cell number on day 3, and embryo quality.
Although mosaicism is frequently observed in blastocysts, the prolonged single culture of blastocysts does not seem to increase the rate of normal cells.
据报道，人类囊胚长时间共培养后非整倍体率会降低。由于共培养在辅助生殖技术中并非常规使用，本研究旨在评估长时间单培养对非整倍体人类胚胎中二倍体细胞率的影响。
在这项队列研究中，我们使用荧光原位杂交（FISH）对受精后第3天接受植入前基因诊断（PGD）的多余囊胚进行重新分析。在受精后的第6天或第7天对它们进行随机研究。
在分析的30个囊胚中，26个（86.6%）观察到嵌合现象，2个（6.7%）为二倍体，2个（6.7%）为三倍体。在嵌合囊胚中，23个（88.5%）被确定为二倍体-非整倍体嵌合，3个（11.5%）为非整倍体嵌合。二倍体细胞超过50%的胚胎总频率为33.3%，与第6天的相关值相比，第7天较低（P<0.05）；然而，根据母亲年龄、囊胚发育阶段、第3天的总细胞数和胚胎质量对胚胎进行分类时，没有差异。
虽然在囊胚中经常观察到嵌合现象，但囊胚长时间单培养似乎不会增加正常细胞率。
