Wang Wen-Tao, Sun Yu-Meng, Huang Wei, He Bo, Zhao Ya-Nan, Chen Yue-Qin
Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory for Biocontrol, School of Life Science, Sun Yat-sen University, Guangzhou 510275, China.
Dept of Obst &Gyn, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China.
Sci Rep. 2016 Mar 18;6:23343. doi: 10.1038/srep23343.
Increasing evidence indicates that long non-coding RNAs (lncRNAs) play important roles in human diseases. This study aimed to investigate the tissue and serum lncRNAs that are differentially expressed between patients with endometriosis, a gynecological disease, to evaluate the potential of these lncRNAs as non-invasive markers for the disease. The differentially expressed lncRNAs as competing endogenous RNAs (ceRNAs) were also analyzed to predict their functions in disease development. Genome-wide profiling of lncRNA expression patterns revealed that many lncRNAs were abnormally expressed between sera and tissuesof the patient samples. A set of aberrant differentially expressed lncRNAs were further validated in a validation cohort of 110 serum and 24 tissue samples. Functional analysis predicted that differentially expressed lncRNAs may participate in disease development through crosstalk between the ceRNAs of miRNAs and may be involved in a range of cellular pathways including steroid or hormone responses. We also found a unique set of lncRNAs that were associated with disease severity and progression, and their diagnostic values were also investigated. Our study demonstrated that lncRNAs could potentially serve as non-invasive biomarkers for the diagnosis of endometriosis and as important regulators in the progression of this disease.
越来越多的证据表明，长链非编码RNA（lncRNA）在人类疾病中发挥着重要作用。本研究旨在调查子宫内膜异位症（一种妇科疾病）患者与健康人之间差异表达的组织和血清lncRNA，以评估这些lncRNA作为该疾病非侵入性标志物的潜力。还分析了作为竞争性内源RNA（ceRNA）的差异表达lncRNA，以预测它们在疾病发展中的功能。lncRNA表达模式的全基因组分析显示，患者样本的血清和组织之间有许多lncRNA异常表达。一组异常差异表达的lncRNA在一个由110份血清和24份组织样本组成的验证队列中得到了进一步验证。功能分析预测，差异表达的lncRNA可能通过miRNA的ceRNA之间的相互作用参与疾病发展，并可能涉及一系列细胞途径，包括类固醇或激素反应。我们还发现了一组与疾病严重程度和进展相关的独特lncRNA，并对它们的诊断价值进行了研究。我们的研究表明，lncRNA有可能作为子宫内膜异位症诊断的非侵入性生物标志物，并作为该疾病进展的重要调节因子。
