Jiao Caifeng, Yang Runqiang, Zhou Yulin, Gu Zhenxin
College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, People's Republic of China.
College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, People's Republic of China.
Food Chem. 2016 Aug 1;204:373-380. doi: 10.1016/j.foodchem.2016.02.147. Epub 2016 Feb 26.
In this study, we investigated the relationships between endogenous NO signal transduction pathways, the antioxidant system and isoflavone accumulation induced by UV-B radiation in soybean sprouts. Results showed that UV-B-triggered NO generation induced isoflavone accumulation by up-regulating the activity and gene expression of key enzymes (phenylalanine ammonia lyase, PAL; chalcone isomerase, CHI; chalcone synthase, CHS; isoflavone synthase, IFS) that participate in isoflavone biosynthesis and enhanced the antioxidant system by regulating levels of antioxidants (glutathione reductase, GR; glutathione S-transferase, GST; ascorbate peroxidase, APX; glutathione GSH; ascorbic acid, ASC), antioxidant enzyme activities (superoxide dismutase, SOD; peroxidase, POD; catalase, CAT) and their gene expression. These effects were inhibited by the addition of a specific NO-scavenger, carboxy-PTIO (cPTIO). The inhibition was reversed through application of the exogenous NO donor, SNP. Overall, NO is an essential signaling molecule, mediating UV-B-induced isoflavone accumulation and the antioxidant system enhancement in soybean sprouts.
在本研究中，我们探究了大豆芽中内源性一氧化氮（NO）信号转导途径、抗氧化系统与UV-B辐射诱导的异黄酮积累之间的关系。结果表明，UV-B引发的NO生成通过上调参与异黄酮生物合成的关键酶（苯丙氨酸解氨酶，PAL；查尔酮异构酶，CHI；查尔酮合酶，CHS；异黄酮合酶，IFS）的活性和基因表达来诱导异黄酮积累，并通过调节抗氧化剂（谷胱甘肽还原酶，GR；谷胱甘肽S-转移酶，GST；抗坏血酸过氧化物酶，APX；谷胱甘肽，GSH；抗坏血酸，ASC）水平、抗氧化酶活性（超氧化物歧化酶，SOD；过氧化物酶，POD；过氧化氢酶，CAT）及其基因表达来增强抗氧化系统。添加特异性NO清除剂羧基-PTIO（cPTIO）可抑制这些效应。通过应用外源NO供体硝普钠（SNP）可逆转这种抑制作用。总体而言，NO是一种重要的信号分子，介导UV-B诱导的大豆芽异黄酮积累和抗氧化系统增强。
