Picot R, Das I, Reid L
Thorax. 1978 Apr;33(2):235-42. doi: 10.1136/thx.33.2.235.
On 100 sputum specimens selected from patients suffering from chronic bronchitis, bronchiectasis, asthma, and cystic fibrosis total deoxyribonucleic acid (DNA) content has been related to macroscopic type, to total dry weight yield, and to the apparent viscosity of the secretion at 1350 s-1: since DNA may be present, either as fibres or within cells, in one-third of the specimens the contribution of each form to the apparent viscosity was assessed. The effect on sputum viscosity of the addition of DNA in vitro has also been studied. Whereas between mucoid, mucopurulent, and purulent macroscopic types a significant difference in total DNA and dry weight yield has been found, viscosity was not significantly correlated with purulence. Similarly, the concentration of either cells or fibres correlated significantly with total DNA but not with viscosity. The in vitro addition of DNA to sputum caused a significant increase in its viscosity, and reasons for the differences between the iv vivo and in vitro effect are discussed. Certain constituents of purulent sputum tend to increase viscosity and others to reduce it, and the influence of these varies in the several diseases studied.
从患有慢性支气管炎、支气管扩张、哮喘和囊性纤维化的患者中选取了100份痰液标本，测定其总脱氧核糖核酸（DNA）含量，并分析其与肉眼可见类型、总干重产量以及分泌物在1350 s-1时的表观粘度之间的关系。由于DNA可能以纤维形式或存在于细胞内，在三分之一的标本中评估了每种形式对表观粘度的贡献。还研究了体外添加DNA对痰液粘度的影响。虽然在粘液样、粘液脓性和脓性这几种肉眼可见类型之间，总DNA和干重产量存在显著差异，但粘度与脓性并无显著相关性。同样，细胞或纤维的浓度与总DNA显著相关，但与粘度无关。体外向痰液中添加DNA会使其粘度显著增加，并对体内和体外效应之间的差异原因进行了讨论。脓性痰液的某些成分倾向于增加粘度，而其他成分则倾向于降低粘度，并且这些成分的影响在几种所研究的疾病中各不相同。
