1 Department of Agricultural and Biosystems Engineering, University of Arizona, Tucson, AZ, USA.
2 Biomedical Engineering Graduate Interdisciplinary Program, University of Arizona, Tucson, AZ, USA.
SLAS Technol. 2017 Feb;22(1):7-12. doi: 10.1177/2211068216639566. Epub 2016 Jul 10.
Use of a smartphone as an optical detector for paper microfluidic devices has recently gained substantial attention due to its simplicity, ease of use, and handheld capability. Utilization of a UV light source enhances the optical signal intensities, especially for the particle immunoagglutination assay that has typically used visible or ambient light. Such enhancement is essential for true assimilation of assays to field deployable and point-of-care applications by greatly reducing the effects by independent environmental factors. This work is the first demonstration of using a UV LED (UVA) to enhance the Mie scatter signals from the particle immunoagglutination assay on the paper microfluidic devices and subsequent smartphone detection. Smartphone's CMOS camera can recognize the UVA scatter from the paper microfluidic channels efficiently in its green channel. For an Escherichia coli assay, the normalized signal intensities increased up to 50% from the negative signal with UV LED, compared with the 4% to 7% with ambient light. Detection limit was 10 colony-forming units/mL. Similar results were obtained in the presence of 10% human whole blood.
由于智能手机具有简单、易用和手持式等特点，将其用作纸微流控设备的光学探测器最近受到了广泛关注。利用紫外光源可以增强光学信号强度，对于通常使用可见光或环境光的粒子免疫凝集检测尤其如此。这种增强对于通过大大减少独立环境因素的影响，真正将检测集成到现场部署和即时护理应用中至关重要。这项工作首次展示了使用紫外发光二极管（UVA）来增强纸上微流控设备上粒子免疫凝集检测的米氏散射信号，并随后通过智能手机进行检测。智能手机的 CMOS 相机可以在其绿色通道中有效地识别来自纸微流道的 UVA 散射。对于大肠杆菌检测，与环境光相比，UV LED 使负信号的归一化信号强度增加了高达 50%，而环境光则增加了 4%至 7%。检测限为 10 个菌落形成单位/毫升。在存在 10%人全血的情况下也得到了类似的结果。
