Lunardi Michele, de Camargo Tozato Claudia, Alfieri Alice Fernandes, de Alcântara Brígida Kussumoto, Vilas-Boas Laurival Antonio, Otonel Rodrigo Alejandro Arellano, Headley Selwyn Arlington, Alfieri Amauri Alcindo
Laboratory of Animal Virology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Londrina, PR, Brazil.
Laboratory of Veterinary Microbiology, Veterinary Teaching Hospital, Universidade de Cuiaba, 3100 Ave Beira Rio, Cuiaba, MT, 78065-900, Brazil.
Arch Virol. 2016 Jun;161(6):1569-77. doi: 10.1007/s00705-016-2820-0. Epub 2016 Mar 21.
Teat papillomatosis affects dairy cows worldwide. Milking can become difficult due to teat warts, and maintaining affected cows in the herds may diminish economic profit in the dairy industry. Currently, 13 bovine papillomavirus (BPV) types have been fully characterized, and numerous putative BPV types have been identified through partial L1 gene PCR. In order to identify the viral types present in warts on the udders of dairy cows, 40 teat lesions from 24 cows from 13 cattle farms in three States of Brazil were evaluated by PV L1 gene PCR. The warts that were evaluated contained sequences from BPVs 6-10, the putative BPV types BAPV9 and BAPV4, and two unreported putative papillomavirus (PV) types, named BPV/BR-UEL6 and BPV/BR-UEL7. In addition, mixed infections and coinfections were identified, since more than one lesion was observed on the udders of 13 cows. Phylogenetic analysis showed that BPV/BR-UEL6 is closely related to BPVs belonging to the genus Xipapillomavirus, while BPV/BR-UEL7 clustered with the previously reported strains Cervus timorensis and Pudu puda PVs, which represent a putative new PV type, and it was only distantly related to xi-, epsilon-, delta- and dyoxi-PVs. These results provide information that will assist in the understanding of the association of BPVs 6, 7, 8, 9, and 10, as well as putative BPV types BAPV4 and BAPV9, with mammary papillomatosis. This is the first characterization of putative novel PV types BPV/BR-UEL6 and BPV/BR-UEL7 in teat warts of dairy cows, highlighting the high genetic diversity of BPVs associated with teat papillomatosis.
乳头瘤病影响着全球的奶牛。由于乳头疣，挤奶可能会变得困难，并且在牛群中饲养患病奶牛可能会降低乳制品行业的经济利润。目前，已完全鉴定出13种牛乳头瘤病毒（BPV）类型，并且通过部分L1基因PCR鉴定出了许多推定的BPV类型。为了鉴定巴西三个州13个奶牛场的24头奶牛乳房上疣中存在的病毒类型，通过PV L1基因PCR对来自这些奶牛的40个乳头病变进行了评估。所评估的疣包含来自BPV 6 - 10、推定的BPV类型BAPV9和BAPV4以及两种未报告的推定乳头瘤病毒（PV）类型的序列，分别命名为BPV/BR-UEL6和BPV/BR-UEL7。此外，由于在13头奶牛的乳房上观察到不止一个病变，因此鉴定出了混合感染和共感染。系统发育分析表明，BPV/BR-UEL6与属于Xipapillomavirus属的BPV密切相关，而BPV/BR-UEL7与先前报道的帝汶鹿和普度鹿PV菌株聚集在一起，代表一种推定的新PV类型，并且它与xi -、epsilon -、delta -和dyoxi - PVs只有远缘关系。这些结果提供的信息将有助于理解BPV 6、7、8、9和10以及推定的BPV类型BAPV4和BAPV9与乳腺乳头瘤病的关联。这是首次在奶牛乳头疣中鉴定推定的新型PV类型BPV/BR-UEL6和BPV/BR-UEL7，突出了与乳头瘤病相关的BPV的高遗传多样性。
