Yue Changwu, Niu Jing, Liu Ning, Lü Yuhong, Liu Minghao, Li Yuanyuan
Guizhou Key Laboratory of Microbial Resources & Drug Development, Zunyi Medical College, Zunyi, Guizhou, China / State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
Guizhou Key Laboratory of Microbial Resources & Drug Development, Zunyi Medical College, Zunyi, Guizhou, China.
Pak J Pharm Sci. 2016 Jan;29(1 Suppl):287-93.
A full length about 105 kb gene cluster containing 35 open reading frames involved in the biosynthesis of lobophorins was cloned and sequenced from a fosmid genomic library of Streptomyces olivaceus strain FXJ7.023. The cluster was identified by genome wide annotation and analysis of secondary metabolite biosynthesis gene clusters by anti SMASH and knockout of loading module-contained region of polyketide skeleton synthesis gene (the starter of lobS1). Gene cluster comparative analysis suggested that the cluster encoded the complete genes for lobophorin polyketide assembly, modification, substrate catalysis, regulation, transportation and resistance, and shows great identity to the newest reported lobophorin biosynthetic gene cluster from Streptomyces sp. SCSIO 01127, but with a significant gene rearrangement in the PKS modules.
从橄榄色链霉菌菌株FXJ7.023的fosmid基因组文库中克隆并测序了一个全长约105 kb的基因簇，该基因簇包含35个参与洛波霉素生物合成的开放阅读框。通过全基因组注释、利用anti SMASH对次级代谢产物生物合成基因簇进行分析以及敲除聚酮骨架合成基因（lobS1的起始物）的负载模块区域来鉴定该基因簇。基因簇比较分析表明，该基因簇编码了洛波霉素聚酮组装、修饰、底物催化、调控、转运和抗性的完整基因，与最新报道的来自链霉菌属SCSIO 01127的洛波霉素生物合成基因簇具有高度同源性，但在聚酮合酶模块中存在明显的基因重排。
