来自链霉菌FJS31-2的新型氯代蒽并苯并恶唑酮类抗生素尊尼霉素B和C，对耐甲氧西林金黄色葡萄球菌和肠球菌具有抗菌活性

Zunyimycins B and C, New Chloroanthrabenzoxocinones Antibiotics against Methicillin-Resistant Staphylococcus aureus and Enterococci from Streptomyces sp. FJS31-2.

作者信息

Lü Yuhong, Shao Meiyun, Wang Yinyin, Qian Shengyan, Wang Miao, Wang Yingquan, Li Xiaoqian, Bao Yuxin, Deng Chengmin, Yue Changwu, Liu Daishun, Liu Ning, Liu Minghao, Huang Ying, Chen Zehui, Hu Yonglin

机构信息

Guizhou Key Laboratory of Microbial Resources & Drug Development, Zunyi Medical University, Zunyi 563003, Guizhou, China.

Zunyi Key Laboratory of Genetic Diagnosis & Targeted Drug Therapy, The First People's Hospital of Zunyi, Zunyi 563003, Guizhou, China.

出版信息

Molecules. 2017 Feb 8;22(2):251. doi: 10.3390/molecules22020251.

This study performed an optimization of the fermentation conditions to activate the expression of the zunyimycin family biosynthesis genes of the zunyimycin-producing streptomycetes strain sp. FJS31-2. Bioassay-guided isolation and purification by varied chromatographic methods yielded two new compounds of the zunyimycin derivatives, namely, 31-2-7 and 31-2-8, accompanied with three known anthrabenzoxocinones family members of zunyimycin A, BE24566B, and chloroanthrabenzoxocinone. Their structures were elucidated by NMR, HRESIMS, IR, UV, and CD. Results showed that these two compounds were structurally similar to the previously reported compound zunyimycin A but differed in positions and number of chlorine atom substitution. The two novel compounds were called zunyimycins B and C. Antibacterial activity assay indicated that zunyimycin C showed a good inhibitory effect on the methicillin-resistant and .

本研究对发酵条件进行了优化，以激活尊尼霉素产生菌链霉菌菌株sp. FJS31-2中尊尼霉素家族生物合成基因的表达。通过生物活性导向，采用多种色谱方法进行分离纯化，得到了两种新的尊尼霉素衍生物化合物，即31-2-7和31-2-8，同时还得到了三种已知的尊尼霉素家族成员，即尊尼霉素A、BE24566B和氯代蒽并苯并恶唑酮。通过核磁共振（NMR）、高分辨电喷雾电离质谱（HRESIMS）、红外光谱（IR）、紫外光谱（UV）和圆二色光谱（CD）对其结构进行了鉴定。结果表明，这两种化合物在结构上与先前报道的化合物尊尼霉素A相似，但氯原子取代的位置和数量有所不同。这两种新化合物被命名为尊尼霉素B和尊尼霉素C。抗菌活性测定表明，尊尼霉素C对耐甲氧西林的……表现出良好的抑制作用。