Lee Young Jin, Ramakrishna Suresh, Chauhan Himanshu, Park Won Sun, Hong Seok-Ho, Kim Kye-Seong
iDream Research Center, MizMedi Women's Hospital, Seoul, 07639 South Korea.
Department of Biomedical Science, Graduate School of Biomedical Science and Engineering, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul, 04763 South Korea.
Cell Regen. 2016 Mar 22;5:2. doi: 10.1186/s13619-016-0028-0. eCollection 2016.
Increasing evidence indicates that microRNAs (miRNAs), endogenous short non-coding RNAs 19-24 nucleotides in length, play key regulatory roles in various biological events at the post-transcriptional level. Embryonic stem cells (ESCs) represent a valuable tool for disease modeling, drug discovery, developmental studies, and potential cell-based therapies in regenerative medicine due to their unlimited self-renewal and pluripotency. Therefore, remarkable progress has been made in recent decades toward understanding the expression and functions of specific miRNAs in the establishment and maintenance of pluripotency. Here, we summarize the recent knowledge regarding the regulatory roles of miRNAs in self-renewal of pluripotent ESCs and during cellular reprogramming, as well as the potential role of miRNAs in two distinct pluripotent states (naïve and primed).
越来越多的证据表明，微小RNA（miRNA），即长度为19 - 24个核苷酸的内源性短非编码RNA，在转录后水平的各种生物学事件中发挥关键调节作用。胚胎干细胞（ESC）由于其无限的自我更新能力和多能性，是疾病建模、药物发现、发育研究以及再生医学中潜在的基于细胞的治疗的宝贵工具。因此，近几十年来在理解特定miRNA在多能性的建立和维持中的表达及功能方面取得了显著进展。在此，我们总结了关于miRNA在多能胚胎干细胞自我更新以及细胞重编程过程中的调节作用的最新知识，以及miRNA在两种不同多能状态（原始态和始发态）中的潜在作用。
