Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, UK.
Roche Pharma Research and Early Development, Roche Innovation Center Basel, Grenzacherstrasse 124, 4070, Basel, Switzerland.
Sci Rep. 2020 Jun 29;10(1):10542. doi: 10.1038/s41598-020-67376-w.
Naïve human pluripotent stem cells (hPSC) resemble the embryonic epiblast at an earlier time-point in development than conventional, 'primed' hPSC. We present a comprehensive miRNA profiling of naïve-to-primed transition in hPSC, a process recapitulating aspects of early in vivo embryogenesis. We identify miR-143-3p and miR-22-3p as markers of the naïve state and miR-363-5p, several members of the miR-17 family, miR-302 family as primed markers. We uncover that miR-371-373 are highly expressed in naïve hPSC. MiR-371-373 are the human homologs of the mouse miR-290 family, which are the most highly expressed miRNAs in naïve mouse PSC. This aligns with the consensus that naïve hPSC resemble mouse naive PSC, showing that the absence of miR-371-373 in conventional hPSC is due to cell state rather than a species difference.
原始人多能干细胞（hPSC）在发育早期比传统的“初始”hPSC 更类似于胚胎外胚层。我们对 hPSC 从原始态到初始态的转变进行了全面的 miRNA 分析，这一过程再现了体内早期胚胎发生的某些方面。我们确定 miR-143-3p 和 miR-22-3p 为原始态的标志物，miR-363-5p、miR-17 家族的几个成员、miR-302 家族为初始态标志物。我们发现 miR-371-373 在原始态 hPSC 中高度表达。miR-371-373 是小鼠 miR-290 家族的人类同源物，在原始态小鼠 PSC 中表达最高。这与原始态 hPSC 类似于小鼠原始态 PSC 的共识一致，表明常规 hPSC 中缺乏 miR-371-373 是由于细胞状态而不是物种差异所致。
