Ruiz Nicolas, Wielgosz-Collin Gaëtane, Poirier Laurence, Grovel Olivier, Petit Karina Ethel, Mohamed-Benkada Mustapha, du Pont Thibaut Robiou, Bissett John, Vérité Philippe, Barnathan Gilles, Pouchus Yves François
Université de Nantes, Nantes Atlantique Universités, SMAB-EA 2160, Faculté de pharmacie, BP 53508, F-44000 Nantes, France.
Peptides. 2007 Jul;28(7):1351-8. doi: 10.1016/j.peptides.2007.05.012. Epub 2007 Jun 6.
A marine strain of Trichoderma longibrachiatum isolated from blue mussels (Mytilus edulis) was investigated for short peptaibol production. Various 11-residue peptaibols, obtained as microheterogenous mixtures after a chromatographic fractionation, were identified by positive mass spectrometry fragmentation (ESI-IT-MS(n), CID-MS(n) and GC/EI-MS). Thirty sequences were identified, which is the largest number of analogous sequences so far observed at once. Twenty-one sequences were new, and nine others corresponded to peptaibols already described. These peptaibols belonged to the same peptidic family based on the model Ac-Aib-xxx-xxx-xxx-Aib-Pro-xxx-xxx-Aib-Pro-xxol. They were named trichobrachin A when the residue in position 2 was an Asn, and trichobrachin C when it was a Gln. Major chromatographic sub-fractions, corresponding to purified peptaibols, were assayed for their cytotoxic activity. Trichobrachin A-IX and trichobrachin C exhibited the highest activities. There was an exponential relation between their relative hydrophobicity and their cytotoxicity on KB cells.
对从蓝贻贝（紫贻贝）中分离出的一株长枝木霉海洋菌株进行了短肽抗生素生产研究。通过色谱分离得到的各种11个残基的肽抗生素，以微异质混合物形式存在，通过正质谱裂解（电喷雾离子阱质谱（ESI-IT-MS(n)）、碰撞诱导解离质谱（CID-MS(n)）和气相色谱/电子轰击质谱（GC/EI-MS））进行鉴定。鉴定出30个序列，这是迄今为止一次观察到的类似序列的最大数量。21个序列是新的，另外9个与已描述的肽抗生素相对应。基于Ac-Aib-xxx-xxx-xxx-Aib-Pro-xxx-xxx-Aib-Pro-xxol模型，这些肽抗生素属于同一肽家族。当第2位的残基为Asn时，它们被命名为曲古菌素A；当为Gln时，则被命名为曲古菌素C。对对应于纯化肽抗生素的主要色谱亚组分进行了细胞毒性活性测定。曲古菌素A-IX和曲古菌素C表现出最高活性。它们的相对疏水性与其对KB细胞的细胞毒性之间存在指数关系。
