Hata Akihisa, Kurosawa Hidetoshi, Endo Yoko, Yamanaka Kenzo, Fujitani Noboru, Endo Ginji
Department of Medical Risk Management, Graduate School of Risk and Crisis Management, Chiba Institute of Science.
J Occup Health. 2016 May 25;58(2):196-200. doi: 10.1539/joh.15-0241-OA. Epub 2016 Mar 24.
The sum of urinary inorganic arsenic (iAs), monomethylarsonic acid (MMA), and dimethylarsinic acid (DMA) concentrations is used for the biological monitoring of occupational iAs exposure. Although DMA is a major metabolite of iAs, it is an inadequate index because high DMA levels are present in urine after seafood consumption. We estimated the urinary iAs+MMA concentration corresponding to iAs exposure.
We used data from two arsenic speciation analyses of urine samples from 330 Bangladeshi with oral iAs exposure and 172 Japanese workers without occupational iAs exposure using high-performance liquid chromatography with inductively coupled plasma mass spectrometry.
iAs, MMA, and DMA, but not arsenobetaine (AsBe), were detected in the urine of the Bangladeshi subjects. The correlation between iAs+MMA+DMA and iAs+MMA was obtained as log (iAs+MMA) = 1.038 log (iAs+MMA+DMA) -0.658. Using the regression formula, the iAs+MMA value was calculated as 2.15 and 7.5 μg As/l, corresponding to 3 and 10 μg As/m(3) of exposures, respectively. In the urine of the Japanese workers, arsenic was mostly excreted as AsBe. We used the 95th percentile of iAs+MMA (12.6 μg As/l) as the background value. The sum of the calculated and background values can be used as a biological indicator of iAs exposure.
We propose 14.8 and 20.1 μg As/l of urinary iAs+MMA as the biological indicators of 3 and 10 μg As/m(3) iAs exposure, respectively.
尿中无机砷（iAs）、一甲基胂酸（MMA）和二甲基胂酸（DMA）浓度之和用于职业性iAs暴露的生物监测。尽管DMA是iAs的主要代谢产物，但它作为指标并不理想，因为食用海鲜后尿中会出现高浓度的DMA。我们估算了与iAs暴露相对应的尿中iAs + MMA浓度。
我们使用高效液相色谱 - 电感耦合等离子体质谱法，对330名有口服iAs暴露的孟加拉国人和172名无职业性iAs暴露的日本工人的尿样进行了两次砷形态分析的数据。
在孟加拉国受试者的尿液中检测到了iAs、MMA和DMA，但未检测到砷甜菜碱（AsBe）。iAs + MMA + DMA与iAs + MMA之间的相关性为log（iAs + MMA）= 1.038 log（iAs + MMA + DMA） - 0.658。使用该回归公式，计算出iAs + MMA值分别为2.15和7.5μg As/l，分别对应3和10μg As/m³的暴露量。在日本工人的尿液中，砷主要以AsBe的形式排出。我们将iAs + MMA的第95百分位数（12.6μg As/l）用作背景值。计算值与背景值之和可作为iAs暴露的生物指标。
我们建议将尿中iAs + MMA浓度分别为14.8和20.1μg As/l作为3和10μg As/m³ iAs暴露的生物指标。
