College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
Department of Marine Life Sciences, Jeju National University, Jeju 63243, Korea.
Mar Drugs. 2021 May 15;19(5):277. doi: 10.3390/md19050277.
In the present study, the in vitro and in vivo anti-inflammatory effects of the sulfated polysaccharides isolated from (SFPS) were evaluated in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages and zebrafish. The results indicated that SFPS improved the viability of LPS-stimulated RAW 264.7 macrophages from 80.02 to 86.80, 90.09, and 94.62% at the concentration of 25, 50, and 100 µg/mL, respectively. Also, SFPS remarkably and concentration-dependently decreased the production levels of inflammatory molecules including nitric oxide (NO), tumor necrosis factor-alpha, prostaglandin E, interleukin-1 beta, and interleukin-6 in LPS-treated RAW 264.7 macrophages. In addition, SFPS significantly inhibited the expression levels of cyclooxygenase-2 and inducible nitric oxide synthase in LPS-treated RAW 264.7 macrophages. Furthermore, the in vivo test results indicated that SFPS improved the survival rate of LPS-treated zebrafish from 53.33 to 56.67, 60.00, and 70.00% at the concentration of 25, 50, and 100 µg/mL, respectively. In addition, SFPS effectively reduced cell death, reactive oxygen species, and NO levels in LPS-stimulated zebrafish. Taken together, these results suggested that SFPS possesses strong in vitro and in vivo anti-inflammatory activities, and could be used as an ingredient to develop anti-inflammatory agents in the functional food and pharmaceutical industries.
在本研究中，评估了从 （SFPS）中分离得到的硫酸多糖的体外和体内抗炎作用在脂多糖（LPS）刺激的 RAW 264.7 巨噬细胞和斑马鱼中。结果表明，SFPS 分别将 LPS 刺激的 RAW 264.7 巨噬细胞的存活率从 80.02 提高到 86.80、90.09 和 94.62%，浓度为 25、50 和 100 µg/mL。此外，SFPS 显著且浓度依赖性地降低了 LPS 处理的 RAW 264.7 巨噬细胞中包括一氧化氮（NO）、肿瘤坏死因子-α、前列腺素 E、白细胞介素-1β和白细胞介素-6 在内的炎症分子的产生水平。此外，SFPS 显著抑制了 LPS 处理的 RAW 264.7 巨噬细胞中环氧化酶-2 和诱导型一氧化氮合酶的表达水平。此外，体内试验结果表明，SFPS 将 LPS 处理的斑马鱼的存活率从 53.33 提高到 56.67、60.00 和 70.00%，浓度分别为 25、50 和 100 µg/mL。此外，SFPS 有效地减少了 LPS 刺激的斑马鱼中的细胞死亡、活性氧和 NO 水平。综上所述，这些结果表明 SFPS 具有很强的体外和体内抗炎活性，可作为功能性食品和制药行业开发抗炎剂的成分。
