Key Laboratory of Medicinal Exploitation and Utilization of Regional Resources, School of Chemical Engineering, Huaiyin Institute of Technology, Huai'an 223003, Jiangsu, PR China; Key Laboratory for Palygorskite Science and Applied Technology of Jiangsu Province, Huaiyin Institute of Technology, Huai'an 223003, Jiangsu, PR China.
Affiliated Huaian Hospital of Xuzhou Medical University, Huai'an 223002, Jiangsu, PR China.
Carbohydr Polym. 2017 Nov 15;176:152-159. doi: 10.1016/j.carbpol.2017.08.073. Epub 2017 Aug 31.
The purpose of this paper was to investigate the anti-inflammatory and anti-angiogenic activities of a purified polysaccharide (CCPS) from flesh of Cipangopaludina chinensis in lipopolysaccharide (LPS) activated RAW264.7 macrophages and human umbilical vein endothelial cells (HUVECs), respectively. Anti-inflammatory results showed that CCPS not only greatly decreased the pro-/anti-inflammatory cytokine secretion ratios, including TNF-α/IL-10, IL-6/IL-10 and IL-1β/IL-10, but also reduced release levels of nitric oxide (NO) and prostaglandin E (PGE), and suppressed expressions of cyclooxygenase2 (COX2) and inducible nitric oxide synthase (iNOS). The results from anti-angiogenic activities revealed that proliferation, migration, tube formation and vascular endothelial growth factor (VEGF) of HUVECs were significantly inhibited by CCPS treatment. Even more remarkable was the fact that anti-inflammatory and anti-angiogenic activities of CCPS exhibited a clear dose-response manner, and these activities were irrelevant to the cytotoxicities of CCPS to RAW264.7 macrophages and HUVECs. These results indicated that CCPS possessed strong anti-inflammatory and anti-angiogenic activities.
本文旨在研究中国圆田螺肉中一种纯化多糖（CCPS）在脂多糖（LPS）激活的 RAW264.7 巨噬细胞和人脐静脉内皮细胞（HUVEC）中的抗炎和抗血管生成活性。抗炎结果表明，CCPS 不仅大大降低了促炎/抗炎细胞因子的分泌比例，包括 TNF-α/IL-10、IL-6/IL-10 和 IL-1β/IL-10，还降低了一氧化氮（NO）和前列腺素 E（PGE）的释放水平，并抑制了环氧化酶 2（COX2）和诱导型一氧化氮合酶（iNOS）的表达。抗血管生成活性的结果表明，CCPS 处理显著抑制了 HUVEC 的增殖、迁移、管形成和血管内皮生长因子（VEGF）。更显著的是，CCPS 的抗炎和抗血管生成活性呈现出明显的剂量反应关系，并且这些活性与 CCPS 对 RAW264.7 巨噬细胞和 HUVEC 的细胞毒性无关。这些结果表明 CCPS 具有很强的抗炎和抗血管生成活性。
